4MBG

Crystal structure of Aspergillus fumigatus protein farnesyltransferase binary complex with farnesyldiphosphate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	CaaX farnesyltransferase alpha subunit Ram2	polymer	367	42391.5	1	UniProt (Q4WP27) Pfam (PF01239) In PDB	Aspergillus fumigatus
2	B	CaaX farnesyltransferase beta subunit Ram1	polymer	519	56635.8	1	UniProt (Q4WPS9) Pfam (PF00432) In PDB	Aspergillus fumigatus
3	A, B	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
4	B	FARNESYL DIPHOSPHATE	non-polymer		382.3	1	Chemie (FPP)
5	B	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
6	B	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
7		water	water		18.0	700	Chemie (HOH)

Sequence modifications

A: 1 - 353 (UniProt: Q4WP27)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Ser 146	Asn 146	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	99027.3
Non-Polymers*	Number of molecules	9
	Total formula weight	859.2
All*	Total formula weight	99886.5

*Water molecules are not included.