4I9T
Structure of the H258Y mutant of the phosphatidylinositol-specific phospholipase C from Staphylococcus aureus
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | 1-phosphatidylinositol phosphodiesterase | polymer | 310 | 35267.2 | 1 | UniProt (P45723) Pfam (PF00388) In PDB | Staphylococcus aureus subsp. aureus | Phosphatidylinositol diacylglycerol-lyase, Phosphatidylinositol-specific phospholipase C, PI-PLC |
2 | A | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
3 | A | 1,2,3,4,5,6-HEXAHYDROXY-CYCLOHEXANE | non-polymer | 180.2 | 2 | Chemie (INS) | |||
4 | A | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 2 | Chemie (PGE) | |||
5 | water | water | 18.0 | 183 | Chemie (HOH) |
Sequence modifications
A: 1 - 302 (UniProt: P45723)
PDB | External Database | Details |
---|---|---|
Tyr 258 | His 268 | engineered mutation |
Leu 303 | - | expression tag |
Glu 304 | - | expression tag |
His 305 | - | expression tag |
His 306 | - | expression tag |
His 307 | - | expression tag |
His 308 | - | expression tag |
His 309 | - | expression tag |
His 310 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 35267.2 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 756.7 | |
All* | Total formula weight | 36023.9 |