4I9J

Structure of the N254Y/H258Y mutant of the phosphatidylinositol-specific phospholipase C from S. aureus bound to diC4PC

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	1-phosphatidylinositol phosphodiesterase	polymer	310	35316.2	1	UniProt (P45723) Pfam (PF00388) In PDB	Staphylococcus aureus subsp. aureus	Phosphatidylinositol diacylglycerol-lyase, Phosphatidylinositol-specific phospholipase C, PI-PLC
2	A	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
3	A	(4S,7R)-7-(heptanoyloxy)-4-hydroxy-N,N,N-trimethyl-10-oxo-3,5,9-trioxa-4-phosphahexadecan-1-aminium 4-oxide	non-polymer		482.6	3	Chemie (XP5)
4		water	water		18.0	230	Chemie (HOH)

Sequence modifications

A: 1 - 302 (UniProt: P45723)

PDB	External Database	Details
Tyr 254	Asn 264	engineered mutation
Tyr 258	His 268	engineered mutation
Leu 303	-	expression tag
Glu 304	-	expression tag
His 305	-	expression tag
His 306	-	expression tag
His 307	-	expression tag
His 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	35316.2
Non-Polymers*	Number of molecules	4
	Total formula weight	1506.7
All*	Total formula weight	36823.0

*Water molecules are not included.