4I68

Crystal structure of the R444A / R449A double mutant of the HERA RNA helicase RRM domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Heat resistant RNA dependent ATPase	polymer	90	9665.1	1	UniProt (Q72GF3) In PDB	Thermus thermophilus	HERA RNA helicase
2	A	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
3	A	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
4		water	water		18.0	111	Chemie (HOH)

Sequence modifications

A: 424 - 510 (UniProt: Q72GF3)

PDB	External Database	Details
Gly 421	-	expression tag
Ala 422	-	expression tag
Met 423	-	expression tag
Ala 444	Arg 451	engineered mutation
Ala 449	Arg 456	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	9665.1
Non-Polymers*	Number of molecules	6
	Total formula weight	302.6
All*	Total formula weight	9967.7

*Water molecules are not included.