4GYJ

Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Uncharacterized lipoprotein ybbD	polymer	648	71230.6	2	UniProt (P40406) Pfam (PF00933) Pfam (PF01915) In PDB	Bacillus subtilis subsp. subtilis	ORF1
2	C, D	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-N-acetyl-alpha-muramic acid	branched		496.5	2	In PDB GlyTouCan (G88499BX)
3	A, B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
4		water	water		18.0	1069	Chemie (HOH)

Sequence modifications

A, B: 18 - 642 (UniProt: P40406)

PDB	External Database	Details
Met -3	-	initiating methionine
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
Ser 7	-	expression tag
Ser 8	-	expression tag
Gly 9	-	expression tag
Leu 10	-	expression tag
Val 11	-	expression tag
Pro 12	-	expression tag
Arg 13	-	expression tag
Gly 14	-	expression tag
Ser 15	-	expression tag
His 16	-	expression tag
Met 17	-	expression tag
Asn 318	Asp 318	engineered mutation
Gly 643	-	expression tag
Ser 644	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	142461.3
Branched	Number of molecules	2
	Total formula weight	992.9
Non-Polymers*	Number of molecules	2
	Total formula weight	442.4
All*	Total formula weight	143896.6

*Water molecules are not included.