4GYF

Crystal structure of histidinol phosphate phosphatase (HISK) from Lactococcus lactis subsp. lactis Il1403 complexed with ZN, L-histidinol and phosphate

Replaces:  3UMU

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Histidinol-phosphatase	polymer	284	33235.8	1	UniProt (Q02150) Pfam (PF02811) In PDB	Lactococcus lactis subsp. lactis	HolPase
2	A	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
3	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
4	A	CHLORIDE ION	non-polymer		35.5	6	Chemie (CL)
5	A	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
6	A	L-histidinol	non-polymer		142.2	2	Chemie (HSO)
7	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
8		water	water		18.0	229	Chemie (HOH)

Sequence modifications

A: 4 - 271 (UniProt: Q02150)

PDB	External Database	Details
Met 1	-	expression tag
Ser 2	-	expression tag
Leu 3	-	expression tag
Gln 65	Arg 63	engineered mutation
Thr 127	Ile 125	engineered mutation
Arg 217	Lys 215	engineered mutation
Lys 272	-	expression tag
Leu 273	-	expression tag
Ala 274	-	expression tag
Ala 275	-	expression tag
Ala 276	-	expression tag
Leu 277	-	expression tag
Glu 278	-	expression tag
His 279	-	expression tag
His 280	-	expression tag
His 281	-	expression tag
His 282	-	expression tag
His 283	-	expression tag
His 284	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	33235.8
Non-Polymers*	Number of molecules	17
	Total formula weight	1195.0
All*	Total formula weight	34430.8

*Water molecules are not included.