4EIQ

Chromopyrrolic acid-soaked RebC-10x with bound 7-carboxy-K252c

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Putative FAD-monooxygenase	polymer	549	59680.3	2	UniProt (Q8KI25) Pfam (PF01494) Pfam (PF21274) In PDB	Lechevalieria aerocolonigenes (Nocardia aerocolonigenes)	Putative monooxygenase, Putative polyketide hydroxylase, RebC
2	A, B	(5S)-7-oxo-6,7,12,13-tetrahydro-5H-indolo[2,3-a]pyrrolo[3,4-c]carbazole-5-carboxylic acid	non-polymer		355.3	2	Chemie (KCT)
3		water	water		18.0	62	Chemie (HOH)

Sequence modifications

A, B: 1 - 529 (UniProt: Q8KI25)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asp 36	Glu 36	engineered mutation
Ala 37	Gln 37	engineered mutation
Gly 38	Thr 38	engineered mutation
Lys 46	Arg 46	engineered mutation
Ser 48	Gly 48	engineered mutation
Ala 117	Gln 117	engineered mutation
Val 216	Phe 216	engineered mutation
Ser 231	Ala 231	engineered mutation
Asn 239	Arg 239	engineered mutation
Val 241	Thr 241	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	119360.7
Non-Polymers*	Number of molecules	2
	Total formula weight	710.7
All*	Total formula weight	120071.4

*Water molecules are not included.