4AFA

Crystal Structure of subtype-switched Epithelial Adhesin 1 to 2 A domain (Epa1to2A) from Candida glabrata in complex with glycerol

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	EPA1P	polymer	262	29372.5	1	UniProt (Q6VBJ0) Pfam (PF10528) In PDB	CANDIDA GLABRATA	EPITHELIAL ADHESIN 1
2	B	beta-D-galactopyranose-(1-3)-alpha-D-glucopyranose	branched		342.3	1	In PDB GlyTouCan (G12058PE)
3	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
4	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5	A	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
6		water	water		18.0	177	Chemie (HOH)

Sequence modifications

A: 31 - 271 (UniProt: Q6VBJ0)

PDB	External Database	Details
Met 10	-	expression tag
Gly 11	-	expression tag
Ser 12	-	expression tag
Ser 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
Ser 20	-	expression tag
Ser 21	-	expression tag
Gly 22	-	expression tag
Leu 23	-	expression tag
Val 24	-	expression tag
Pro 25	-	expression tag
Arg 26	-	expression tag
Gly 27	-	expression tag
Ser 28	-	expression tag
His 29	-	expression tag
Met 30	-	expression tag
Asp 227	Glu 227	engineered mutation
Asn 228	Tyr 228	engineered mutation
Asn 229	Asp 229	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	29372.5
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	5
	Total formula weight	422.5
All*	Total formula weight	30137.3

*Water molecules are not included.