3WXF

Crystal structure of CYLD USP domain (C596S E674Q) in complex with Met1-linked diubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Uncharacterized protein	polymer	312	35990.5	2	UniProt (E7FEV5) Pfam (PF00443) In PDB	Danio rerio (zebra fish)	CYLD protein
2	B, D	Ubiquitin	polymer	148	16751.2	2	UniProt (P0CG48) Pfam (PF00240) In PDB	Homo sapiens (human)	M1-linked diubiquitin
3	C	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4		water	water		18.0	147	Chemie (HOH)

Sequence modifications

A, C: 578 - 780 (UniProt: E7FEV5)

PDB	External Database	Details
Ser 575	-	expression tag
His 576	-	expression tag
Met 577	-	expression tag
Ser 596	Cys 596	engineered mutation
Gln 674	Glu 674	engineered mutation

A, C: 785 - 886 (UniProt: E7FEV5)

PDB	External Database	Details
Leu 781	-	linker
Glu 782	-	linker
Gly 783	-	linker
Gly 784	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	105483.5
Non-Polymers*	Number of molecules	2
	Total formula weight	192.1
All*	Total formula weight	105675.6

*Water molecules are not included.