3WN5
Crystal structure of asymmetrically engineered Fc variant in complex with FcgRIIIa
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D | Ig gamma-1 chain C region | polymer | 230 | 25850.2 | 2 | UniProt (P01857) Pfam (PF07654) In PDB | Homo sapiens (human) | |
| 2 | B, E | Ig gamma-1 chain C region | polymer | 230 | 26227.6 | 2 | UniProt (P01857) Pfam (PF07654) In PDB | Homo sapiens (human) | |
| 3 | C, F | Low affinity immunoglobulin gamma Fc region receptor III-A | polymer | 197 | 22626.3 | 2 | UniProt (P08637) Pfam (PF13895) In PDB | Homo sapiens (human) | CD16a antigen, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2 |
| 4 | G, H, L | beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1625.5 | 3 | In PDB GlyTouCan (G33244HE) | |||
| 5 | I | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 6 | J, M | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 2 | In PDB GlyTouCan (G42666HT) | |||
| 7 | K | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1463.3 | 1 | In PDB GlyTouCan (G59451NL) | |||
| 8 | F, A, B, C, D... | IODIDE ION | non-polymer | 126.9 | 16 | Chemie (IOD) | |||
| 9 | F | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
| 10 | water | water | 18.0 | 41 | Chemie (HOH) |
Sequence modifications
A, D: 216 - 445 (UniProt: P01857)
B, E: 216 - 445 (UniProt: P01857)
C, F: -3 - 187 (UniProt: P08637)
| PDB | External Database | Details |
|---|---|---|
| Ser 220 | Cys 103 | engineered mutation |
| Glu 270 | Asp 153 | engineered mutation |
| Asp 326 | Lys 209 | engineered mutation |
| Lys 330 | Ala 213 | engineered mutation |
| Glu 334 | Lys 217 | engineered mutation |
| Cys 356 | Asp 239 | engineered mutation |
| Ser 366 | Thr 249 | engineered mutation |
| Ala 368 | Leu 251 | engineered mutation |
| Val 407 | Tyr 290 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser 220 | Cys 103 | engineered mutation |
| Tyr 234 | Leu 117 | engineered mutation |
| Tyr 235 | Leu 118 | engineered mutation |
| Trp 236 | Gly 119 | engineered mutation |
| Met 239 | Ser 122 | engineered mutation |
| Asp 268 | His 151 | engineered mutation |
| Ala 298 | Ser 181 | engineered mutation |
| Asp 327 | Ala 210 | engineered mutation |
| Cys 349 | Tyr 232 | engineered mutation |
| Trp 366 | Thr 249 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gln 35 | Asn 56 | engineered mutation |
| Gln 71 | Asn 92 | engineered mutation |
| Val 155 | Phe 176 | SEE REMARK 999 |
| Gln 166 | Asn 187 | engineered mutation |
| His 188 | - | expression tag |
| His 189 | - | expression tag |
| His 190 | - | expression tag |
| His 191 | - | expression tag |
| His 192 | - | expression tag |
| His 193 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 149408.2 | |
| Branched | Number of molecules | 7 |
| Total formula weight | 8099.4 | |
| Non-Polymers* | Number of molecules | 17 |
| Total formula weight | 2251.7 | |
| All* | Total formula weight | 159759.3 |
