3WG5

1510-N membrane-bound stomatin-specific protease K138A mutant in complex with a substrate peptide under heat treatment

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	441aa long hypothetical nfeD protein	polymer	230	25368.0	2	UniProt (O59179) Pfam (PF00574) In PDB	Pyrococcus horikoshii	1510-N membrane protease
2	C	PH1511 stomatin	polymer	10	1158.5	1	UniProt (O59180) In PDB	Pyrococcus horikoshii OT3	Uncharacterized protein PH1511
3	A, B	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	A, B	IMIDAZOLE	non-polymer		69.1	3	Chemie (IMD)
5	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6		water	water		18.0	140	Chemie (HOH)

Sequence modifications

A, B: 16 - 236 (UniProt: O59179)

PDB	External Database	Details
Met 15	-	expression tag
Ala 138	Lys 138	engineered mutation
Leu 237	-	expression tag
Glu 238	-	expression tag
His 239	-	expression tag
His 240	-	expression tag
His 241	-	expression tag
His 242	-	expression tag
His 243	-	expression tag
His 244	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	51894.5
Non-Polymers*	Number of molecules	6
	Total formula weight	426.9
All*	Total formula weight	52321.4

*Water molecules are not included.