3QOL
Crystal structure of Staphylococcal nuclease variant D+PHS/V23E at pH 6 determined at 100 K
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Thermonuclease | polymer | 143 | 16173.4 | 1 | UniProt (P00644) Pfam (PF00565) UniProt (by SIFTS) (Q8NXI6) In PDB | Staphylococcus aureus | TNase, Micrococcal nuclease, Staphylococcal nuclease, Nuclease B, Nuclease A |
2 | A | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
3 | A | THYMIDINE-3',5'-DIPHOSPHATE | non-polymer | 402.2 | 1 | Chemie (THP) | |||
4 | water | water | 18.0 | 76 | Chemie (HOH) |
Sequence modifications
A: 1 - 149 (UniProt: P00644)
PDB | External Database | Details |
---|---|---|
Glu 23 | Val 63 | engineered mutation |
- | Thr 84 | deletion |
- | Lys 85 | deletion |
- | His 86 | deletion |
- | Pro 87 | deletion |
- | Lys 88 | deletion |
- | Lys 89 | deletion |
Phe 50 | Gly 90 | engineered mutation |
Asn 51 | Val 91 | engineered mutation |
Gly 117 | Pro 157 | engineered mutation |
Leu 124 | His 164 | engineered mutation |
Ala 128 | Ser 168 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 16173.4 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 442.3 | |
All* | Total formula weight | 16615.7 |