3Q23

X-ray crystal structure of the N4 mini-VRNAP and P2_7a promoter transcription initiation complex with GMPCPP and Manganese: sustrate complex II

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Virion RNA polymerase	polymer	1118	123193.5	2	UniProt (Q859P9) Pfam (PF21624) Pfam (PF21769) In PDB	Enterobacteria phage N4 (Bacteriophage N4)
2	C, D	DNA (5'-D(*TP*GP*CP*CP*TP*CP*CP*CP*AP*GP*GP*CP*AP*TP*CP*CP*AP*AP*AP*AP*GP*AP*AP*GP*CP*GP*GP*AP*GP*CP*TP*TP*CP*TP*TP*C)-3')	polymer	36	11007.1	2
3	A, B	PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER	non-polymer		521.2	4	Chemie (G2P)
4	A, B	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
5	A, B	DIHYDROGENPHOSPHATE ION	non-polymer		97.0	2	Chemie (2HP)
6		water	water		18.0	2626	Chemie (HOH)

Sequence modifications

A, B: 1 - 1106 (UniProt: Q859P9)

PDB	External Database	Details
Met -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Arg -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	268401.2
Non-Polymers*	Number of molecules	10
	Total formula weight	2498.6
All*	Total formula weight	270899.7

*Water molecules are not included.