3OY9

Crystal structure of the Prototype Foamy Virus (PFV) intasome in complex with manganese at 2.55 resolution

Replaces:  3L2S

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	PFV integrase	polymer	395	44456.7	2	UniProt (P14350) Pfam (PF17921) Pfam (PF00665) Pfam (PF18103) In PDB	Human spumaretrovirus (SFVcpz(hu))	p42In
2	C	DNA (5'-D(*AP*TP*TP*GP*TP*CP*AP*TP*GP*GP*AP*AP*TP*TP*TP*CP*GP*CP*A)-3')	polymer	19	5834.8	1
3	D	DNA (5'-D(*TP*GP*CP*GP*AP*AP*AP*TP*TP*CP*CP*AP*TP*GP*AP*CP*A)-3')	polymer	17	5195.4	1
4	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	A, B	MANGANESE (II) ION	non-polymer		54.9	3	Chemie (MN)
6	A, B	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
7	A	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
8	A	AMMONIUM ION	non-polymer		18.0	1	Chemie (NH4)
9		water	water		18.0	274	Chemie (HOH)

Sequence modifications

A, B: 1 - 392 (UniProt: P14350)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag
Ser 217	Gly 968	variant
Gly 218	Ser 969	variant

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	99943.6
Non-Polymers*	Number of molecules	11
	Total formula weight	808.8
All*	Total formula weight	100752.3

*Water molecules are not included.