3OSQ
Maltose-bound maltose sensor engineered by insertion of circularly permuted green fluorescent protein into E. coli maltose binding protein at position 175
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Maltose-binding periplasmic protein,Green fluorescent protein | polymer | 663 | 73705.0 | 1 | UniProt (P0AEY0) UniProt (P42212) Pfam (PF01547) Pfam (PF01353) In PDB | Escherichia coli O157:H7 | MBP,MMBP,Maltodextrin-binding protein |
2 | B | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
3 | A | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
4 | water | water | 18.0 | 294 | Chemie (HOH) |
Sequence modifications
A: 1 - 173 (UniProt: P0AEY0)
A: 176 - 267 (UniProt: P42212)
A: 276 - 420 (UniProt: P42212)
A: 422 - 618 (UniProt: P0AEY0)
PDB | External Database | Details |
---|---|---|
Met -35 | - | expression tag |
Arg -34 | - | expression tag |
Gly -33 | - | expression tag |
Ser -32 | - | expression tag |
His -31 | - | expression tag |
His -30 | - | expression tag |
His -29 | - | expression tag |
His -28 | - | expression tag |
His -27 | - | expression tag |
His -26 | - | expression tag |
Gly -25 | - | expression tag |
Met -24 | - | expression tag |
Ala -23 | - | expression tag |
Ser -22 | - | expression tag |
Met -21 | - | expression tag |
Thr -20 | - | expression tag |
Gly -19 | - | expression tag |
Gly -18 | - | expression tag |
Gln -17 | - | expression tag |
Gln -16 | - | expression tag |
Met -15 | - | expression tag |
Gly -14 | - | expression tag |
Arg -13 | - | expression tag |
Asp -12 | - | expression tag |
Leu -11 | - | expression tag |
Tyr -10 | - | expression tag |
Asp -9 | - | expression tag |
Asp -8 | - | expression tag |
Asp -7 | - | expression tag |
Asp -6 | - | expression tag |
Lys -5 | - | expression tag |
Asp -4 | - | expression tag |
Arg -3 | - | expression tag |
Trp -2 | - | expression tag |
Gly -1 | - | expression tag |
Ser 0 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Ala 192 | Val 163 | conflict |
Gly 204 | Ser 175 | conflict |
Tyr 209 | Asp 180 | conflict |
Lys 235 | Ala 206 | conflict |
Leu 260 | His 231 | conflict |
PDB | External Database | Details |
---|---|---|
Gly 268 | - | linker |
Gly 269 | - | linker |
Thr 270 | - | linker |
Gly 271 | - | linker |
Gly 272 | - | linker |
Ser 273 | - | linker |
Met 274 | - | linker |
Val 275 | - | linker |
Leu 338 | Phe 64 | conflict |
C12 340 | Ser 65 | chromophore |
C12 340 | Tyr 66 | chromophore |
C12 340 | Gly 67 | chromophore |
Ile 367 | Val 93 | conflict |
Phe 419 | Tyr 145 | conflict |
Gly 421 | - | linker |
PDB | External Database | Details |
---|---|---|
His 174 | - | linker |
Leu 175 | - | linker |
Val 560 | Ala 338 | conflict |
Asp 608 | Ala 386 | conflict |
Gly 619 | - | expression tag |
Ser 620 | - | expression tag |
His 621 | - | expression tag |
His 622 | - | expression tag |
His 623 | - | expression tag |
His 624 | - | expression tag |
His 625 | - | expression tag |
His 626 | - | expression tag |
Gly 627 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 73705.0 | |
Branched | Number of molecules | 1 |
Total formula weight | 342.3 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 192.1 | |
All* | Total formula weight | 74239.4 |