3OOR

I-SceI mutant (K86R/G100T)complexed with C/G+4 DNA substrate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Intron encoded endonuclease I-SceI	polymer	237	28058.6	1	UniProt (P03882) Pfam (PF03161) In PDB	Saccharomyces cerevisiae (yeast)	21S rRNA intron maturase, Homing endonuclease omega
2	C	5'-D(*CP*AP*CP*GP*CP*TP*AP*GP*GP*GP*AP*TP*AP*AP*CP*CP*GP*GP*GP*TP*AP*AP*TP*AP*C)-3'	polymer	25	7717.0	1
3	D	5'-D(*GP*GP*TP*AP*TP*TP*AP*CP*CP*CP*GP*GP*TP*TP*AP*TP*CP*CP*CP*TP*AP*GP*CP*GP*T)-3'	polymer	25	7640.9	1
4	A, D	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
5		water	water		18.0	11	Chemie (HOH)

Sequence modifications

A: 1 - 235 (UniProt: P03882)

PDB	External Database	Details
Arg 86	Lys 86	engineered mutation
Thr 100	Gly 100	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	43416.5
Non-Polymers*	Number of molecules	3
	Total formula weight	120.2
All*	Total formula weight	43536.8

*Water molecules are not included.