3NVJ

Crystal structure of the C143A/C166A mutant of Ero1p

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Endoplasmic oxidoreductin-1	polymer	393	45294.9	1	UniProt (Q03103) Pfam (PF04137) In PDB	Saccharomyces cerevisiae (yeast)	Ero1p, endoplasmic oxidoreductase protein 1
2	A	1-ETHYL-PYRROLIDINE-2,5-DIONE	non-polymer		127.1	1	Chemie (NEN)
3	A	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	1	Chemie (FAD)
4	A	CADMIUM ION	non-polymer		112.4	1	Chemie (CD)
5		water	water		18.0	12	Chemie (HOH)

Sequence modifications

A: 56 - 424 (UniProt: Q03103)

PDB	External Database	Details
Gly 39	-	expression tag
Ser 40	-	expression tag
His 41	-	expression tag
His 42	-	expression tag
His 43	-	expression tag
His 44	-	expression tag
His 45	-	expression tag
His 46	-	expression tag
Ser 47	-	expression tag
Ser 48	-	expression tag
Gly 49	-	expression tag
Leu 50	-	expression tag
Val 51	-	expression tag
Pro 52	-	expression tag
Arg 53	-	expression tag
Gly 54	-	expression tag
Ser 55	-	expression tag
Ala 143	Cys 143	engineered mutation
Ala 166	Cys 166	engineered mutation
Leu 425	-	expression tag
Glu 426	-	expression tag
Arg 427	-	expression tag
Pro 428	-	expression tag
His 429	-	expression tag
Arg 430	-	expression tag
Asp 431	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	45294.9
Non-Polymers*	Number of molecules	3
	Total formula weight	1025.1
All*	Total formula weight	46320.0

*Water molecules are not included.