3NSY

The multi-copper oxidase CueO with six Met to Ser mutations (M358S,M361S,M362S,M364S,M366S,M368S)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Blue copper oxidase cueO	polymer	511	55749.3	1	UniProt (P36649) Pfam (PF07732) Pfam (PF00394) Pfam (PF07731) In PDB	Escherichia coli
2	A	COPPER (II) ION	non-polymer		63.5	4	Chemie (CU)
3	A	CU-O-CU LINKAGE	non-polymer		143.1	1	Chemie (C2O)
4		water	water		18.0	305	Chemie (HOH)

Sequence modifications

A: 29 - 516 (UniProt: P36649)

PDB	External Database	Details
Ser 358	Met 358	engineered mutation
Ser 361	Met 361	engineered mutation
Ser 362	Met 362	engineered mutation
Ser 364	Met 364	engineered mutation
Ser 366	Met 366	engineered mutation
Ser 368	Met 368	engineered mutation
Leu 517	-	expression tag
Gln 518	-	expression tag
Gly 519	-	expression tag
Asp 520	-	expression tag
His 521	-	expression tag
Gly 522	-	expression tag
Leu 523	-	expression tag
Gln 524	-	expression tag
Gly 525	-	expression tag
Asp 526	-	expression tag
His 527	-	expression tag
Gly 528	-	expression tag
Leu 529	-	expression tag
Ser 530	-	expression tag
Ala 531	-	expression tag
Trp 532	-	expression tag
Ser 533	-	expression tag
His 534	-	expression tag
Pro 535	-	expression tag
Gln 536	-	expression tag
Phe 537	-	expression tag
Glu 538	-	expression tag
Lys 539	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	55749.3
Non-Polymers*	Number of molecules	5
	Total formula weight	397.3
All*	Total formula weight	56146.6

*Water molecules are not included.