3MIS

I-MsoI re-designed for altered DNA cleavage specificity (-8G)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Mso-8G	polymer	161	18630.4	2		synthetic construct (artificial gene)
2	C	DNA (5'-D(*GP*CP*AP*GP*GP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*CP*TP*CP*CP*G)-3')	polymer	24	7420.8	1
3	D	DNA (5'-D(*CP*GP*GP*AP*GP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*CP*CP*TP*GP*C)-3')	polymer	24	7322.7	1
4	A, B, C	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
5		water	water		18.0	93	Chemie (HOH)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	52004.3
Non-Polymers*	Number of molecules	3
	Total formula weight	120.2
All*	Total formula weight	52124.6

*Water molecules are not included.