3JYT

K65R mutant HIV-1 reverse transcriptase cross-linked to DS-DNA and complexed with DATP as the incoming nucleotide substrate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Reverse transcriptase/ribonuclease H	polymer	558	64277.7	1	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075) In PDB	Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1)	Exoribonuclease H,p66 RT
2	B	p51 RT	polymer	437	51095.6	1	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) In PDB	Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1)
3	T	DNA (5'-D(*A*TP*GP*GP*TP*CP*GP*GP*CP*GP*CP*CP*CP*GP*AP*AP*CP*AP*GP*GP*GP*AP*CP*TP*GP*TP*G)-3')	polymer	27	8383.4	1		synthetic construct
4	P	DNA (5'-D(*A*CP*AP*GP*TP*CP*CP*CP*TP*GP*TP*TP*CP*GP*GP*(MRG)P*CP*GP*CP*CP*(DDG))-3')	polymer	21	6474.3	1		synthetic construct
5	A	2'-DEOXYADENOSINE 5'-TRIPHOSPHATE	non-polymer		491.2	1	Chemie (DTP)
6	A	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
7	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A: 1 - 558 (UniProt: P03366)

PDB	External Database	Details
Arg 65	Lys 664	engineered mutation
Cys 258	Gln 857	engineered mutation
Ser 280	Cys 879	engineered mutation

B: 1 - 429 (UniProt: P03366)

PDB	External Database	Details
Ser 280	Cys 879	engineered mutation
Gly 430	-	expression tag
Gly 431	-	expression tag
His 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag
His 436	-	expression tag
His 437	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	130230.9
Non-Polymers*	Number of molecules	5
	Total formula weight	731.9
All*	Total formula weight	130962.9

*Water molecules are not included.