3HGO

Crystal structure of the F74Y/H244Y OPR3 double mutant from tomato

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	12-oxophytodienoate reductase 3	polymer	402	44442.2	2	UniProt (Q9FEW9) Pfam (PF00724) In PDB	Solanum lycopersicum (tomato)	12-oxophytodienoate-10,11-reductase 3, OPDA-reductase 3, LeOPR3
2	A, B	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	2	Chemie (FMN)
3		water	water		18.0	501	Chemie (HOH)

Sequence modifications

A, B: 1 - 396 (UniProt: Q9FEW9)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Tyr 74	Phe 74	engineered mutation
Tyr 244	His 244	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	88884.5
Non-Polymers*	Number of molecules	2
	Total formula weight	912.7
All*	Total formula weight	89797.1

*Water molecules are not included.