3EKH

Calcium-saturated GCaMP2 T116V/K378W mutant monomer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Myosin light chain kinase, Green fluorescent protein, Calmodulin chimera	polymer	451	50759.6	1	UniProt (P42212) UniProt (P0DP29) Pfam (PF01353) Pfam (PF13499) UniProt (by SIFTS) (P11799) In PDB	artificial gene
2	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
3	A	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
4		water	water		18.0	277	Chemie (HOH)

Sequence modifications

A: 1 - 61 (PDB: 3EKH)

PDB	External Database	Details
Met 1	-	initiating methionine

A: 62 - 151 (UniProt: P42212)

PDB	External Database	Details
Ala 76	Val 163	see remark 999
Gly 88	Ser 175	see remark 999
Tyr 93	Asp 180	see remark 999
Val 116	Thr 203	engineered mutation
Lys 119	Ala 206	see remark 999
Leu 144	His 231	see remark 999

A: 160 - 302 (UniProt: P42212)

PDB	External Database	Details
Gly 152	-	linker
Gly 153	-	linker
Thr 154	-	linker
Gly 155	-	linker
Gly 156	-	linker
Ser 157	-	linker
Met 158	-	linker
Val 159	-	linker
Leu 222	Phe 64	see remark 999
Cro 224	Ser 65	chromophore
-	Tyr 66	deletion
-	Gly 67	deletion
Ile 251	Val 93	see remark 999
Thr 303	-	linker
Arg 304	-	linker

A: 305 - 451 (UniProt: P0DP29)

PDB	External Database	Details
Trp 378	Lys 76	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	50759.6
Non-Polymers*	Number of molecules	5
	Total formula weight	252.4
All*	Total formula weight	51012.1

*Water molecules are not included.