2Y6Z

Crystallographic structure of GM23 an example of Catalytic migration from TIM to thiamin phosphate synthase.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	TRIOSE-PHOSPHATE ISOMERASE	polymer	256	27158.0	1	UniProt (P04789) Pfam (PF00121) In PDB	TRYPANOSOMA BRUCEI BRUCEI	GM23, TIM
2	A	THIAMIN PHOSPHATE	non-polymer		345.3	1	Chemie (TPS)
3	A	PYROPHOSPHATE 2-	non-polymer		176.0	1	Chemie (POP)
4		water	water		18.0	85	Chemie (HOH)

Sequence modifications

A: 21 - 265 (UniProt: P04789)

PDB	External Database	Details
Ser 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
Leu 14	-	expression tag
Val 15	-	expression tag
Pro 16	-	expression tag
Arg 17	-	expression tag
Gly 18	-	expression tag
Ser 19	-	expression tag
Ala 20	-	expression tag
Gly 22	Ser 2	engineered mutation
Gly 37	Ser 17	engineered mutation
Gly 38	Gln 18	engineered mutation
Pro 63	Ser 43	engineered mutation
Ser 64	Thr 44	engineered mutation
Trp 65	Phe 45	engineered mutation
Tyr 66	Val 46	engineered mutation
Met 67	His 47	engineered mutation
Gly 68	-	insertion
Ala 69	-	insertion
Gln 70	Leu 48	engineered mutation
Gly 90	Ile 68	engineered mutation
Asn 91	Ala 69	engineered mutation
Ala 92	Lys 70	engineered mutation
Asp 93	Ser 71	engineered mutation
-	Gly 72	deletion
-	Ala 73	deletion
-	Phe 74	deletion
-	Thr 75	deletion
-	Gly 76	deletion
-	Glu 77	deletion
-	Val 78	deletion
Ala 94	Ser 79	engineered mutation
Ala 96	Pro 81	engineered mutation
Ser 97	Ile 82	engineered mutation
Ile 103	Val 88	engineered mutation
Ser 104	Asn 89	engineered mutation
Ser 150	Glu 135	engineered mutation
Thr 163	Ala 148	engineered mutation
Val 213	Ile 198	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27158.0
Non-Polymers*	Number of molecules	2
	Total formula weight	521.3
All*	Total formula weight	27679.3

*Water molecules are not included.