2XJE

Crystal structure of the D52N variant of cytosolic 5'-nucleotidase II in complex with uridine 5'-monophosphate and adenosine triphosphate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	CYTOSOLIC PURINE 5'-NUCLEOTIDASE	polymer	555	64087.9	1	UniProt (P49902) Pfam (PF05761) In PDB	HOMO SAPIENS (HUMAN)	CYTOSOLIC 5'-NUCLEOTIDASE II
2	A	URIDINE-5'-MONOPHOSPHATE	non-polymer		324.2	1	Chemie (U5P)
3	A	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
4	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6		water	water		18.0	177	Chemie (HOH)

Sequence modifications

A: 1 - 536 (UniProt: P49902)

PDB	External Database	Details
Met -18	-	expression tag
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Asn 52	Asp 52	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	64087.9
Non-Polymers*	Number of molecules	5
	Total formula weight	972.1
All*	Total formula weight	65060.0

*Water molecules are not included.