2QCX

Crystal structure of Bacillus subtilis TenA Y112F mutant complexed with formyl aminomethyl pyrimidine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Transcriptional activator tenA	polymer	263	30554.0	2	UniProt (P25052) Pfam (PF03070) In PDB	Bacillus subtilis	Thiaminase II, Thiaminase-2
2	A, B	N-[(4-AMINO-2-METHYLPYRIMIDIN-5-YL)METHYL]-N-(2-HYDROXYETHYL)FORMAMIDE	non-polymer		210.2	2	Chemie (PF1)
3		water	water		18.0	106	Chemie (HOH)

Sequence modifications

A, B: 1 - 236 (UniProt: P25052)

PDB	External Database	Details
Met -26	-	expression tag
Gly -25	-	expression tag
Ser -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
Asp -17	-	expression tag
Ile -16	-	expression tag
Thr -15	-	expression tag
Ser -14	-	expression tag
Leu -13	-	expression tag
Tyr -12	-	expression tag
Lys -11	-	expression tag
Lys -10	-	expression tag
Ala -9	-	expression tag
Gly -8	-	expression tag
Ser -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Phe 112	Tyr 112	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	61108.0
Non-Polymers*	Number of molecules	2
	Total formula weight	420.5
All*	Total formula weight	61528.5

*Water molecules are not included.