2IVQ

SITE DIRECTED MUTAGENESIS OF KEY RESIDUES INVOLVED IN THE CATALYTIC MECHANISM OF CYANASE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	CYANATE HYDRATASE	polymer	156	17039.8	10	UniProt (P00816) Pfam (PF21291) Pfam (PF02560) In PDB	ESCHERICHIA COLI	CYANASE LYASE, CYANASE, CYANATE HYDROLASE
2	F, G, A, H, B...	SULFATE ION	non-polymer		96.1	24	Chemie (SO4)
3	F, G, H, A, I...	CHLORIDE ION	non-polymer		35.5	13	Chemie (CL)
4		water	water		18.0	1124	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H, I, J: 1 - 156 (UniProt: P00816)

PDB	External Database	Details
Lys 96	Arg 96	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	170397.8
Non-Polymers*	Number of molecules	37
	Total formula weight	2766.4
All*	Total formula weight	173164.2

*Water molecules are not included.