2IV1

SITE DIRECTED MUTAGENESIS OF KEY RESIDUES INVOLVED IN THE CATALYTIC MECHANISM OF CYANASE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	CYANATE HYDRATASE	polymer	156	17038.7	10	UniProt (P00816) Pfam (PF21291) Pfam (PF02560) In PDB	ESCHERICHIA COLI	CYANASE LYASE, CYANASE, CYANATE HYDROLASE
2	F, G, H, A, I...	SULFATE ION	non-polymer		96.1	22	Chemie (SO4)
3	F, G, H, I, A...	CHLORIDE ION	non-polymer		35.5	10	Chemie (CL)
4		water	water		18.0	2469	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H, I, J: 1 - 156 (UniProt: P00816)

PDB	External Database	Details
Gln 96	Arg 96	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	170387.3
Non-Polymers*	Number of molecules	32
	Total formula weight	2467.9
All*	Total formula weight	172855.2

*Water molecules are not included.