2GFT
Crystal structure of the E263A nucleophile mutant of Bacillus licheniformis endo-beta-1,4-galactanase in complex with galactotriose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Glycosyl Hydrolase Family 53 | polymer | 399 | 43720.4 | 2 | GenBank (52005769) Pfam (PF07745) UniProt (by SIFTS) (Q65CX5) In PDB | Bacillus licheniformis | |
| 2 | C, D | beta-D-galactopyranose-(1-4)-beta-D-galactopyranose-(1-4)-beta-D-galactopyranose | branched | 504.4 | 2 | In PDB BIRD (PRD_900114) GlyTouCan (G06996VN) | 4beta-beta-galactotriose | ||
| 3 | A, B | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 4 | water | water | 18.0 | 294 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 399 (GenBank: 52005769)
| PDB | External Database | Details |
|---|---|---|
| His 2 | Ala 28 | conflict |
| Ala 263 | Glu 289 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 87440.8 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1008.9 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 80.2 | |
| All* | Total formula weight | 88529.8 |
