2G8I
B. halodurans RNase H catalytic domain D192N mutant in complex with Mn2+ and RNA/DNA hybrid (non-P nick at the active site)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | B | 5'-R(*UP*CP*GP*AP*CP*A)-3' | polymer | 6 | 1875.2 | 1 | |||
| 2 | C | 5'-D(*AP*TP*GP*TP*CP*G)-3' | polymer | 6 | 1824.2 | 1 | |||
| 3 | A | Ribonuclease H | polymer | 142 | 16329.5 | 1 | UniProt (Q9KEI9) In PDB | Bacillus halodurans | RNase H |
| 4 | A | MANGANESE (II) ION | non-polymer | 54.9 | 4 | Chemie (MN) | |||
| 5 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 6 | A | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 1 | Chemie (MPD) | |||
| 7 | water | water | 18.0 | 331 | Chemie (HOH) |
Sequence modifications
A: 59 - 196 (UniProt: Q9KEI9)
| PDB | External Database | Details |
|---|---|---|
| Gly 55 | - | cloning artifact |
| Ser 56 | - | cloning artifact |
| His 57 | - | cloning artifact |
| Met 58 | - | cloning artifact |
| Asn 192 | Asp 192 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 20028.9 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 373.4 | |
| All* | Total formula weight | 20402.3 |
