2F8N

2.9 Angstrom X-ray structure of hybrid macroH2A nucleosomes

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	I, J	alpha-satellite DNA (146 bp)	polymer	146	45054.8	2		Homo sapiens (human)
2	A, E	Histone H3.1	polymer	136	15421.1	2	UniProt (P84233) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)
3	B, F	Histone H4	polymer	103	11394.4	2	UniProt (P62799) Pfam (PF15511) In PDB	Xenopus laevis (African clawed frog)
4	D	Histone 3, H2ba	polymer	126	14025.3	1	UniProt (Q9D2U9) Pfam (PF00125) In PDB	Mus musculus (house mouse)
5	H	Histone H2B.1	polymer	123	13655.9	1	UniProt (P02281) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)
6	G	Core histone macro-H2A.1	polymer	120	12984.3	1	UniProt (O75367) Pfam (PF00125) Pfam (PF16211) In PDB	Homo sapiens (human)
7	K	Histone H2A type 1	polymer	149	16198.8	1	UniProt (Q8CGP6) Pfam (PF00125) Pfam (PF16211) In PDB	Mus musculus (house mouse)
8		water	water		18.0	120	Chemie (HOH)

Sequence modifications

H: 1401 - 1522 (UniProt: P02281)

PDB	External Database	Details
Met 1400	-	initiating methionine
Thr 1429	Ser 32	conflict

G: 1003 - 1122 (UniProt: O75367)

PDB	External Database	Details
Val 1067	Gly 64	conflict

K: 0 - 129 (UniProt: Q8CGP6)

PDB	External Database	Details
Met -19	-	cloning artifact
Gly -18	-	cloning artifact
Ser -17	-	cloning artifact
Ser -16	-	cloning artifact
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	cloning artifact
Ser -8	-	cloning artifact
Gly -7	-	cloning artifact
Leu -6	-	cloning artifact
Val -5	-	cloning artifact
Pro -4	-	cloning artifact
Arg -3	-	cloning artifact
Gly -2	-	cloning artifact
Ser -1	-	cloning artifact

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	200605.1
All*	Total formula weight	200605.1

*Water molecules are not included.