2BG7
Bacillus cereus metallo-beta-lactamase (BcII) Arg (121) Cys mutant. Solved at pH4.5 using 20 Micromolar ZnSO4 in the buffer. 1mM DTT was used as a reducing agent. Cys221 is oxidized.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | BETA-LACTAMASE II | polymer | 227 | 24957.5 | 2 | UniProt (P04190) Pfam (PF00753) In PDB | BACILLUS CEREUS | METALLO-BETA-LACTAMASE, PENICILLINASE, CEPHALOSPORINASE |
| 2 | A, B | GLYCEROL | non-polymer | 92.1 | 12 | Chemie (GOL) | |||
| 3 | A, B | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 4 | A, B | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) | |||
| 5 | water | water | 18.0 | 348 | Chemie (HOH) |
Sequence modifications
A, B: 209 - 211 (UniProt: P04190)
| PDB | External Database | Details |
|---|---|---|
| Cys 121 | Arg 121 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 49915.0 | |
| Non-Polymers* | Number of molecules | 18 |
| Total formula weight | 1620.2 | |
| All* | Total formula weight | 51535.2 |
