1ZP4

Glu28Gln mutant of E. coli Methylenetetrahydrofolate Reductase (oxidized) complex with Methyltetrahydrofolate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	5,10-methylenetetrahydrofolate reductase	polymer	304	34214.9	3	UniProt (P00394) Pfam (PF02219) UniProt (by SIFTS) (P0AEZ1) In PDB	Escherichia coli
2	A, C	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3	A, B, C	5-METHYL-5,6,7,8-TETRAHYDROFOLIC ACID	non-polymer		459.5	3	Chemie (C2F)
4	A, B, C	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	3	Chemie (FAD)
5		water	water		18.0	509	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 296 (UniProt: P00394)

PDB	External Database	Details
Gln 28	Glu 28	engineered mutation
Leu 297	-	cloning artifact
Glu 298	-	cloning artifact
His 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag
His 302	-	expression tag
His 303	-	expression tag
His 304	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	102644.6
Non-Polymers*	Number of molecules	8
	Total formula weight	3927.1
All*	Total formula weight	106571.7

*Water molecules are not included.