1Y5L
The crystal structure of the NarGHI mutant NarI-H66Y
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Respiratory nitrate reductase 1 alpha chain | polymer | 1246 | 140526.8 | 1 | UniProt (P09152) Pfam (PF14710) Pfam (PF00384) Pfam (PF01568) In PDB | Escherichia coli | NarG, Nitrate reductase A alpha subunit, Quinol-nitrate oxidoreductase alpha subunit |
2 | B | Respiratory nitrate reductase 1 beta chain | polymer | 512 | 58140.3 | 1 | UniProt (P11349) Pfam (PF13247) Pfam (PF14711) In PDB | Escherichia coli | NarH, Nitrate reductase A beta subunit, Quinol-nitrate oxidoreductase beta subunit |
3 | C | Respiratory nitrate reductase 1 gamma chain | polymer | 225 | 25577.4 | 1 | UniProt (P11350) Pfam (PF02665) In PDB | Escherichia coli | NarI, Nitrate reductase A gamma subunit, Quinol-nitrate oxidoreductase gamma subunit, Cytochrome B-NR |
4 | A | PHOSPHORIC ACID 4-(2-AMINO-4-OXO-3,4,5,6,-TETRAHYDRO-PTERIDIN-6-YL)-2-HYDROXY-3,4-DIMERCAPTO-BUT-3-EN-YL ESTER GUANYLATE ESTER | non-polymer | 740.6 | 2 | Chemie (MD1) | |||
5 | A | MOLYBDENUM(VI) ION | non-polymer | 95.9 | 1 | Chemie (6MO) | |||
6 | A, B | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 4 | Chemie (SF4) | |||
7 | A | 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE | non-polymer | 705.0 | 1 | Chemie (3PH) | |||
8 | B | FE3-S4 CLUSTER | non-polymer | 295.8 | 1 | Chemie (F3S) | |||
9 | C | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 1 | Chemie (HEM) | |||
10 | water | water | 18.0 | 351 | Chemie (HOH) |
Sequence modifications
C: 1 - 225 (UniProt: P11350)
PDB | External Database | Details |
---|---|---|
Fme 1 | Met 1 | modified residue |
Tyr 66 | His 66 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 224244.5 | |
Non-Polymers* | Number of molecules | 10 |
Total formula weight | 4600.9 | |
All* | Total formula weight | 228845.4 |