1Y5I

The crystal structure of the NarGHI mutant NarI-K86A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Respiratory nitrate reductase 1 alpha chain	polymer	1246	140526.8	1	UniProt (P09152) Pfam (PF14710) Pfam (PF00384) Pfam (PF01568) In PDB	Escherichia coli	NarG, Nitrate reductase A alpha subunit, Quinol-nitrate oxidoreductase alpha subunit
2	B	Respiratory nitrate reductase 1 beta chain	polymer	512	58140.3	1	UniProt (P11349) Pfam (PF13247) Pfam (PF14711) In PDB	Escherichia coli	NarH, Nitrate reductase A beta subunit, Quinol-nitrate oxidoreductase beta subunit
3	C	Respiratory nitrate reductase 1 gamma chain	polymer	225	25494.3	1	UniProt (P11350) Pfam (PF02665) In PDB	Escherichia coli	NarI, Nitrate reductase A gamma subunit, Quinol-nitrate oxidoreductase gamma subunit, Cytochrome B-NR
4	A	PHOSPHORIC ACID 4-(2-AMINO-4-OXO-3,4,5,6,-TETRAHYDRO-PTERIDIN-6-YL)-2-HYDROXY-3,4-DIMERCAPTO-BUT-3-EN-YL ESTER GUANYLATE ESTER	non-polymer		740.6	2	Chemie (MD1)
5	A	MOLYBDENUM(VI) ION	non-polymer		95.9	1	Chemie (6MO)
6	A, B	IRON/SULFUR CLUSTER	non-polymer		351.6	4	Chemie (SF4)
7	B	FE3-S4 CLUSTER	non-polymer		295.8	1	Chemie (F3S)
8	B	1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE	non-polymer		705.0	1	Chemie (3PH)
9	C	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	2	Chemie (HEM)
10	C	(1S)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTANOYLOXY)METHYL]ETHYL OCTANOATE	non-polymer		455.5	1	Chemie (AGA)
11		water	water		18.0	1118	Chemie (HOH)

Sequence modifications

C: 1 - 225 (UniProt: P11350)

PDB	External Database	Details
Fme 1	Met 1	modified residue
Ala 86	Lys 86	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	224161.3
Non-Polymers*	Number of molecules	12
	Total formula weight	5672.8
All*	Total formula weight	229834.2

*Water molecules are not included.