1XMZ

Crystal structure of the dark state of kindling fluorescent protein kfp from anemonia sulcata

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	GFP-like non-fluorescent chromoprotein FP595 chain 1	polymer	74	8065.2	2	UniProt (Q9GZ28) In PDB	Anemonia sulcata (snake-locks sea anemone)
2	C, D	GFP-like non-fluorescent chromoprotein FP595 chain 2	polymer	170	19127.7	2	UniProt (Q9GZ28) Pfam (PF01353) In PDB	Anemonia sulcata (snake-locks sea anemone)
3	C, D	BETA-MERCAPTOETHANOL	non-polymer		78.1	7	Chemie (BME)
4		water	water		18.0	463	Chemie (HOH)

Sequence modifications

A, B: 2 - 62 (UniProt: Q9GZ28)

PDB	External Database	Details
Met -10	-	initiating methionine
Arg -9	-	expression tag
Gly -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Nh2 63	-	cloning artifact

C, D: 65 - 232 (UniProt: Q9GZ28)

PDB	External Database	Details
Crk 65	Met 63	chromophore
Crk 65	Tyr 64	chromophore
Crk 65	Gly 65	chromophore
Gly 143	Ala 143	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	54385.7
Non-Polymers*	Number of molecules	7
	Total formula weight	546.9
All*	Total formula weight	54932.7

*Water molecules are not included.