1TL9

High resolution crystal structure of calpain I protease core in complex with leupeptin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Calpain 1, large [catalytic] subunit	polymer	339	38804.6	1	UniProt (P97571) Pfam (PF00648) In PDB	Rattus norvegicus (Norway rat)	Calcium-activated neutral proteinase, CANP, Mu-type, muCANP, Micromolar-calpain
2	B	leupeptin inhibitor	polymer	4	429.6	1	NOR (NOR00487) BIRD (PRD_000216)	Actinomycetes Streptomyces roseus MA 839-A1
3	A	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4		water	water		18.0	287	Chemie (HOH)

Sequence modifications

A: 27 - 356 (UniProt: P97571)

PDB	External Database	Details
Met 26	-	initiating methionine
Leu 357	-	expression tag
Glu 358	-	expression tag
His 359	-	expression tag
His 360	-	expression tag
His 361	-	expression tag
His 362	-	expression tag
His 363	-	expression tag
His 364	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	39234.1
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	39314.3

*Water molecules are not included.