1OJN

SPECIFICITY AND MECHANISM OF STREPTOCOCCUS PNEUMONIAE HYALURONATE LYASE: COMPLEX OF THE TYR408PHE MUTANT WITH 6-SULPHATED CHONDROITIN DISACCHARIDE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	HYALURONATE LYASE	polymer	731	83545.9	1	UniProt (Q54873) Pfam (PF08124) Pfam (PF02278) Pfam (PF02884) In PDB	STREPTOCOCCUS PNEUMONIAE	HYALURONIDASE, HYASE
2	B	4-deoxy-alpha-L-threo-hex-4-enopyranuronic acid-(1-3)-2-acetamido-2-deoxy-6-O-sulfo-beta-D-galactopyranose	branched		459.4	1	In PDB GlyTouCan (G73490KK)
3	A	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
4		water	water		18.0	638	Chemie (HOH)

Sequence modifications

A: 168 - 892 (UniProt: Q54873)

PDB	External Database	Details
His 893	-	expression tag
His 894	-	expression tag
His 895	-	expression tag
His 896	-	expression tag
His 897	-	expression tag
His 898	-	expression tag
Thr 173	Ala 290	conflict
Asp 196	Glu 313	conflict
Ile 223	Thr 340	conflict
Phe 408	Tyr 525	conflict
Arg 496	Cys 613	conflict
Thr 541	Pro 658	conflict
Ser 704	Gly 821	conflict
Ser 736	Phe 853	conflict
Gly 790	Arg 907	conflict

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	83545.9
Branched	Number of molecules	1
	Total formula weight	459.4
Non-Polymers*	Number of molecules	4
	Total formula weight	384.3
All*	Total formula weight	84389.6

*Water molecules are not included.