1I2C

CRYSTAL STRUCTURE OF MUTANT T145A SQD1 PROTEIN COMPLEX WITH NAD AND UDP-GLUCOSE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	SULFOLIPID BIOSYNTHESIS PROTEIN SQD1	polymer	404	45479.5	1	UniProt (O48917) Pfam (PF01370) In PDB	Arabidopsis thaliana (thale cress)
2	A	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3	A	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	1	Chemie (NAD)
4	A	URIDINE-5'-DIPHOSPHATE-GLUCOSE	non-polymer		566.3	1	Chemie (UPG)
5		water	water		18.0	398	Chemie (HOH)

Sequence modifications

A: 3 - 394 (UniProt: O48917)

PDB	External Database	Details
Met -9	-	expression tag
Arg -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Gly 1	-	expression tag
Ser 2	-	expression tag
Ala 145	Thr 228	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	45479.5
Non-Polymers*	Number of molecules	4
	Total formula weight	1421.9
All*	Total formula weight	46901.4

*Water molecules are not included.