1E9F

Mutant human thymidylate kinase complexed with TMP and ADP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	THYMIDYLATE KINASE	polymer	217	24223.7	1	UniProt (P23919) UniProt (P37345) Pfam (PF02223) UniProt (by SIFTS) (P0A720) In PDB	HOMO SAPIENS	DTMP KINASE
2	A	THYMIDINE-5'-PHOSPHATE	non-polymer		322.2	1	Chemie (TMP)
3	A	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
4	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5		water	water		18.0	162	Chemie (HOH)

Sequence modifications

A: 1 - 144 (UniProt: P23919)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gly 16	Arg 16	engineered mutation

A: 149 - 212 (UniProt: P23919)

PDB	External Database	Details
Ala 200	Arg 200	engineered mutation
Ile 184	Leu 184	conflict
Ile 191	Leu 191	conflict
Asp 190	Glu 190	conflict
Ser 183	Arg 183	conflict

A: 145 - 148 (UniProt: P37345)

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	24223.7
Non-Polymers*	Number of molecules	4
	Total formula weight	798.0
All*	Total formula weight	25021.7

*Water molecules are not included.