8IQF

Cryo-EM structure of the dimeric human CAF1-H3-H4 complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000785	cellular_component	chromatin
A	0003682	molecular_function	chromatin binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0006260	biological_process	DNA replication
A	0006281	biological_process	DNA repair
A	0006334	biological_process	nucleosome assembly
A	0006335	biological_process	DNA replication-dependent chromatin assembly
A	0032991	cellular_component	protein-containing complex
A	0033186	cellular_component	CAF-1 complex
A	0042802	molecular_function	identical protein binding
A	0051082	molecular_function	unfolded protein binding
A	0070087	molecular_function	chromo shadow domain binding
B	0000785	cellular_component	chromatin
B	0003682	molecular_function	chromatin binding
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006260	biological_process	DNA replication
B	0006281	biological_process	DNA repair
B	0006334	biological_process	nucleosome assembly
B	0006335	biological_process	DNA replication-dependent chromatin assembly
B	0032991	cellular_component	protein-containing complex
B	0033186	cellular_component	CAF-1 complex
B	0042393	molecular_function	histone binding
B	0051082	molecular_function	unfolded protein binding
C	0000118	cellular_component	histone deacetylase complex
C	0000122	biological_process	negative regulation of transcription by RNA polymerase II
C	0000781	cellular_component	chromosome, telomeric region
C	0000785	cellular_component	chromatin
C	0000978	molecular_function	RNA polymerase II cis-regulatory region sequence-specific DNA binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005694	cellular_component	chromosome
C	0005829	cellular_component	cytosol
C	0006260	biological_process	DNA replication
C	0006325	biological_process	chromatin organization
C	0006334	biological_process	nucleosome assembly
C	0006335	biological_process	DNA replication-dependent chromatin assembly
C	0006338	biological_process	chromatin remodeling
C	0006355	biological_process	regulation of DNA-templated transcription
C	0007420	biological_process	brain development
C	0008094	molecular_function	ATP-dependent activity, acting on DNA
C	0008285	biological_process	negative regulation of cell population proliferation
C	0016581	cellular_component	NuRD complex
C	0016589	cellular_component	NURF complex
C	0030336	biological_process	negative regulation of cell migration
C	0030512	biological_process	negative regulation of transforming growth factor beta receptor signaling pathway
C	0031492	molecular_function	nucleosomal DNA binding
C	0032991	cellular_component	protein-containing complex
C	0033186	cellular_component	CAF-1 complex
C	0035098	cellular_component	ESC/E(Z) complex
C	0042393	molecular_function	histone binding
C	0042659	biological_process	regulation of cell fate specification
C	0042826	molecular_function	histone deacetylase binding
C	0045892	biological_process	negative regulation of DNA-templated transcription
C	0045893	biological_process	positive regulation of DNA-templated transcription
C	0070822	cellular_component	Sin3-type complex
C	1902455	biological_process	negative regulation of stem cell population maintenance
C	1902459	biological_process	positive regulation of stem cell population maintenance
C	1904949	cellular_component	ATPase complex
C	2000736	biological_process	regulation of stem cell differentiation
D	0000786	cellular_component	nucleosome
D	0003677	molecular_function	DNA binding
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005694	cellular_component	chromosome
D	0006325	biological_process	chromatin organization
D	0006334	biological_process	nucleosome assembly
D	0010467	biological_process	gene expression
D	0016020	cellular_component	membrane
D	0030527	molecular_function	structural constituent of chromatin
D	0032200	biological_process	telomere organization
D	0032991	cellular_component	protein-containing complex
D	0040029	biological_process	epigenetic regulation of gene expression
D	0043229	cellular_component	intracellular organelle
D	0045296	molecular_function	cadherin binding
D	0046982	molecular_function	protein heterodimerization activity
D	0070062	cellular_component	extracellular exosome
E	0000781	cellular_component	chromosome, telomeric region
E	0000786	cellular_component	nucleosome
E	0003677	molecular_function	DNA binding
E	0003723	molecular_function	RNA binding
E	0005515	molecular_function	protein binding
E	0005576	cellular_component	extracellular region
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005694	cellular_component	chromosome
E	0006325	biological_process	chromatin organization
E	0006334	biological_process	nucleosome assembly
E	0016020	cellular_component	membrane
E	0030527	molecular_function	structural constituent of chromatin
E	0032200	biological_process	telomere organization
E	0032991	cellular_component	protein-containing complex
E	0043505	cellular_component	CENP-A containing nucleosome
E	0045653	biological_process	negative regulation of megakaryocyte differentiation
E	0046982	molecular_function	protein heterodimerization activity
E	0061644	biological_process	protein localization to CENP-A containing chromatin
E	0070062	cellular_component	extracellular exosome
F	0000785	cellular_component	chromatin
F	0003682	molecular_function	chromatin binding
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0006260	biological_process	DNA replication
F	0006281	biological_process	DNA repair
F	0006334	biological_process	nucleosome assembly
F	0006335	biological_process	DNA replication-dependent chromatin assembly
F	0032991	cellular_component	protein-containing complex
F	0033186	cellular_component	CAF-1 complex
F	0042802	molecular_function	identical protein binding
F	0051082	molecular_function	unfolded protein binding
F	0070087	molecular_function	chromo shadow domain binding
G	0000785	cellular_component	chromatin
G	0003682	molecular_function	chromatin binding
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006260	biological_process	DNA replication
G	0006281	biological_process	DNA repair
G	0006334	biological_process	nucleosome assembly
G	0006335	biological_process	DNA replication-dependent chromatin assembly
G	0032991	cellular_component	protein-containing complex
G	0033186	cellular_component	CAF-1 complex
G	0042393	molecular_function	histone binding
G	0051082	molecular_function	unfolded protein binding
H	0000118	cellular_component	histone deacetylase complex
H	0000122	biological_process	negative regulation of transcription by RNA polymerase II
H	0000781	cellular_component	chromosome, telomeric region
H	0000785	cellular_component	chromatin
H	0000978	molecular_function	RNA polymerase II cis-regulatory region sequence-specific DNA binding
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005694	cellular_component	chromosome
H	0005829	cellular_component	cytosol
H	0006260	biological_process	DNA replication
H	0006325	biological_process	chromatin organization
H	0006334	biological_process	nucleosome assembly
H	0006335	biological_process	DNA replication-dependent chromatin assembly
H	0006338	biological_process	chromatin remodeling
H	0006355	biological_process	regulation of DNA-templated transcription
H	0007420	biological_process	brain development
H	0008094	molecular_function	ATP-dependent activity, acting on DNA
H	0008285	biological_process	negative regulation of cell population proliferation
H	0016581	cellular_component	NuRD complex
H	0016589	cellular_component	NURF complex
H	0030336	biological_process	negative regulation of cell migration
H	0030512	biological_process	negative regulation of transforming growth factor beta receptor signaling pathway
H	0031492	molecular_function	nucleosomal DNA binding
H	0032991	cellular_component	protein-containing complex
H	0033186	cellular_component	CAF-1 complex
H	0035098	cellular_component	ESC/E(Z) complex
H	0042393	molecular_function	histone binding
H	0042659	biological_process	regulation of cell fate specification
H	0042826	molecular_function	histone deacetylase binding
H	0045892	biological_process	negative regulation of DNA-templated transcription
H	0045893	biological_process	positive regulation of DNA-templated transcription
H	0070822	cellular_component	Sin3-type complex
H	1902455	biological_process	negative regulation of stem cell population maintenance
H	1902459	biological_process	positive regulation of stem cell population maintenance
H	1904949	cellular_component	ATPase complex
H	2000736	biological_process	regulation of stem cell differentiation
I	0000786	cellular_component	nucleosome
I	0003677	molecular_function	DNA binding
I	0005515	molecular_function	protein binding
I	0005576	cellular_component	extracellular region
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005694	cellular_component	chromosome
I	0006325	biological_process	chromatin organization
I	0006334	biological_process	nucleosome assembly
I	0010467	biological_process	gene expression
I	0016020	cellular_component	membrane
I	0030527	molecular_function	structural constituent of chromatin
I	0032200	biological_process	telomere organization
I	0032991	cellular_component	protein-containing complex
I	0040029	biological_process	epigenetic regulation of gene expression
I	0043229	cellular_component	intracellular organelle
I	0045296	molecular_function	cadherin binding
I	0046982	molecular_function	protein heterodimerization activity
I	0070062	cellular_component	extracellular exosome
J	0000781	cellular_component	chromosome, telomeric region
J	0000786	cellular_component	nucleosome
J	0003677	molecular_function	DNA binding
J	0003723	molecular_function	RNA binding
J	0005515	molecular_function	protein binding
J	0005576	cellular_component	extracellular region
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005694	cellular_component	chromosome
J	0006325	biological_process	chromatin organization
J	0006334	biological_process	nucleosome assembly
J	0016020	cellular_component	membrane
J	0030527	molecular_function	structural constituent of chromatin
J	0032200	biological_process	telomere organization
J	0032991	cellular_component	protein-containing complex
J	0043505	cellular_component	CENP-A containing nucleosome
J	0045653	biological_process	negative regulation of megakaryocyte differentiation
J	0046982	molecular_function	protein heterodimerization activity
J	0061644	biological_process	protein localization to CENP-A containing chromatin
J	0070062	cellular_component	extracellular exosome

Functional Information from PROSITE/UniProt

site_id	PS00322
Number of Residues	7
Details	HISTONE_H3_1 Histone H3 signature 1. KAPRKQL

Chain	Residue	Details
D	LYS14-LEU20

---

site_id	PS00959
Number of Residues	9
Details	HISTONE_H3_2 Histone H3 signature 2. PFqRLVREI

Chain	Residue	Details
D	PRO66-ILE74

---

site_id	PS00047
Number of Residues	5
Details	HISTONE_H4 Histone H4 signature. GAKRH

Chain	Residue	Details
E	GLY14-HIS18

---

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. LLSAsdDhTICLWDI

Chain	Residue	Details
C	LEU289-LEU303
C	LEU333-LEU347
B	MET144-VAL158
C	LEU193-ILE207

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
B	THR394
G	THR394

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231

Chain	Residue	Details
B	SER409
G	SER409
F	SER123
F	SER138

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163

Chain	Residue	Details
B	THR419
B	THR433
G	THR419
G	THR433

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:23186163

Chain	Residue	Details
B	SER429
G	SER429

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163

Chain	Residue	Details
B	SER458
G	SER458
E	LYS44
J	LYS8
J	LYS16
J	LYS44

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
B	LYS494
G	LYS494
E	LYS77
E	LYS91
J	LYS12
J	LYS31
J	LYS77
J	LYS91

---

site_id	SWS_FT_FI7
Number of Residues	8
Details	MOD_RES: Phosphothreonine => ECO:0000255

Chain	Residue	Details
B	THR495
B	THR509
B	THR521
B	THR531
G	THR495
G	THR509
G	THR521
G	THR531

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648

Chain	Residue	Details
B	SER538
G	SER538

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:15592455, ECO:0007744|PubMed:20068231

Chain	Residue	Details
E	TYR51
J	TYR51

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	MOD_RES: N6-glutaryllysine; alternate => ECO:0000269|PubMed:31542297

Chain	Residue	Details
E	LYS59
J	LYS59
I	LYS14
I	LYS56

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:P62806

Chain	Residue	Details
E	LYS79
J	LYS79

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P62806

Chain	Residue	Details
E	THR80
J	THR80
I	LYS18
I	LYS64

---

site_id	SWS_FT_FI13
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:24275569

Chain	Residue	Details
E	TYR88
J	TYR88

---

site_id	SWS_FT_FI14
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744|PubMed:25218447

Chain	Residue	Details
E	LYS12
F	LYS182
J	LYS12

---

site_id	SWS_FT_FI15
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000269|PubMed:19818714

Chain	Residue	Details
D	LYS27
E	LYS91
J	LYS91

---

site_id	SWS_FT_FI16
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744|PubMed:28112733

Chain	Residue	Details
E	LYS20
E	LYS59
E	LYS79
J	LYS20
J	LYS59
J	LYS79

---

site_id	SWS_FT_FI17
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in UFM1); alternate => ECO:0000269|PubMed:30886146

Chain	Residue	Details
E	LYS31
J	LYS31

---

site_id	SWS_FT_FI18
Number of Residues	2
Details	MOD_RES: N6-methyllysine => ECO:0000269|PubMed:15983376

Chain	Residue	Details
D	LYS37
I	LYS37

---

site_id	SWS_FT_FI19
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000269|PubMed:19783980

Chain	Residue	Details
D	TYR41
I	TYR41

---

site_id	SWS_FT_FI20
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:20850016

Chain	Residue	Details
D	SER57
I	SER57

---

site_id	SWS_FT_FI21
Number of Residues	2
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000269|PubMed:22389435, ECO:0000269|PubMed:29211711

Chain	Residue	Details
D	LYS79
I	LYS79

---

site_id	SWS_FT_FI22
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0000269|PubMed:20850016

Chain	Residue	Details
D	THR80
I	THR80

---

site_id	SWS_FT_FI23
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P84243

Chain	Residue	Details
D	SER86
I	SER86

---

site_id	SWS_FT_FI24
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:Q71DI3

Chain	Residue	Details
D	THR107
I	THR107

---

site_id	SWS_FT_FI25
Number of Residues	2
Details	MOD_RES: N6-glutaryllysine; alternate => ECO:0000269|PubMed:31542297

Chain	Residue	Details
D	LYS115
I	LYS115

---

site_id	SWS_FT_FI26
Number of Residues	2
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000269|PubMed:22389435, ECO:0000269|PubMed:27436229

Chain	Residue	Details
D	LYS122
I	LYS122

---

site_id	SWS_FT_FI27
Number of Residues	2
Details	LIPID: N6-decanoyllysine => ECO:0000269|PubMed:35939806

Chain	Residue	Details
D	LYS18
I	LYS18

---