8H7G

Cryo-EM structure of the human SAGA complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000124	cellular_component	SAGA complex
A	0000375	biological_process	RNA splicing, via transesterification reactions
A	0000398	biological_process	mRNA splicing, via spliceosome
A	0003676	molecular_function	nucleic acid binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005681	cellular_component	spliceosomal complex
A	0005684	cellular_component	U2-type spliceosomal complex
A	0005686	cellular_component	U2 snRNP
A	0005689	cellular_component	U12-type spliceosomal complex
A	0005730	cellular_component	nucleolus
A	0006282	biological_process	regulation of DNA repair
A	0006397	biological_process	mRNA processing
A	0008380	biological_process	RNA splicing
A	0030620	molecular_function	U2 snRNA binding
A	0042177	biological_process	negative regulation of protein catabolic process
A	0043484	biological_process	regulation of RNA splicing
A	0044877	molecular_function	protein-containing complex binding
A	0045893	biological_process	positive regulation of DNA-templated transcription
A	0071005	cellular_component	U2-type precatalytic spliceosome
A	0071013	cellular_component	catalytic step 2 spliceosome
A	1903241	biological_process	U2-type prespliceosome assembly
B	0000124	cellular_component	SAGA complex
B	0000398	biological_process	mRNA splicing, via spliceosome
B	0003723	molecular_function	RNA binding
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005681	cellular_component	spliceosomal complex
B	0005684	cellular_component	U2-type spliceosomal complex
B	0005686	cellular_component	U2 snRNP
B	0005689	cellular_component	U12-type spliceosomal complex
B	0006282	biological_process	regulation of DNA repair
B	0006397	biological_process	mRNA processing
B	0008380	biological_process	RNA splicing
B	0043484	biological_process	regulation of RNA splicing
B	0045893	biological_process	positive regulation of DNA-templated transcription
B	0071005	cellular_component	U2-type precatalytic spliceosome
B	0071011	cellular_component	precatalytic spliceosome
B	1903241	biological_process	U2-type prespliceosome assembly
B	1990935	molecular_function	splicing factor binding
C	0000124	cellular_component	SAGA complex
C	0000786	cellular_component	nucleosome
C	0000812	cellular_component	Swr1 complex
C	0003712	molecular_function	transcription coregulator activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005794	cellular_component	Golgi apparatus
C	0006281	biological_process	DNA repair
C	0006282	biological_process	regulation of DNA repair
C	0006325	biological_process	chromatin organization
C	0006355	biological_process	regulation of DNA-templated transcription
C	0006357	biological_process	regulation of transcription by RNA polymerase II
C	0016301	molecular_function	kinase activity
C	0033276	cellular_component	transcription factor TFTC complex
C	0035267	cellular_component	NuA4 histone acetyltransferase complex
C	0042981	biological_process	regulation of apoptotic process
C	0043484	biological_process	regulation of RNA splicing
C	0045893	biological_process	positive regulation of DNA-templated transcription
C	0051171	biological_process	obsolete regulation of nitrogen compound metabolic process
C	0051726	biological_process	regulation of cell cycle
C	0080090	biological_process	regulation of primary metabolic process
C	0080135	biological_process	regulation of cellular response to stress
C	1905168	biological_process	positive regulation of double-strand break repair via homologous recombination
C	2000779	biological_process	regulation of double-strand break repair
D	0000124	cellular_component	SAGA complex
D	0003712	molecular_function	transcription coregulator activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0006282	biological_process	regulation of DNA repair
D	0006357	biological_process	regulation of transcription by RNA polymerase II
D	0006914	biological_process	autophagy
D	0007369	biological_process	gastrulation
D	0043484	biological_process	regulation of RNA splicing
D	0045893	biological_process	positive regulation of DNA-templated transcription
D	0070461	cellular_component	SAGA-type complex
E	0000124	cellular_component	SAGA complex
E	0003713	molecular_function	transcription coactivator activity
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0006282	biological_process	regulation of DNA repair
E	0006355	biological_process	regulation of DNA-templated transcription
E	0006357	biological_process	regulation of transcription by RNA polymerase II
E	0006366	biological_process	transcription by RNA polymerase II
E	0033276	cellular_component	transcription factor TFTC complex
E	0043229	cellular_component	intracellular organelle
E	0043484	biological_process	regulation of RNA splicing
E	0045893	biological_process	positive regulation of DNA-templated transcription
E	0046982	molecular_function	protein heterodimerization activity
G	0000124	cellular_component	SAGA complex
G	0003713	molecular_function	transcription coactivator activity
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005829	cellular_component	cytosol
G	0005925	cellular_component	focal adhesion
G	0006282	biological_process	regulation of DNA repair
G	0006357	biological_process	regulation of transcription by RNA polymerase II
G	0043484	biological_process	regulation of RNA splicing
G	0045893	biological_process	positive regulation of DNA-templated transcription
G	0070461	cellular_component	SAGA-type complex
H	0000123	cellular_component	histone acetyltransferase complex
H	0000124	cellular_component	SAGA complex
H	0003713	molecular_function	transcription coactivator activity
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005669	cellular_component	transcription factor TFIID complex
H	0006282	biological_process	regulation of DNA repair
H	0006355	biological_process	regulation of DNA-templated transcription
H	0006357	biological_process	regulation of transcription by RNA polymerase II
H	0006366	biological_process	transcription by RNA polymerase II
H	0016607	cellular_component	nuclear speck
H	0033276	cellular_component	transcription factor TFTC complex
H	0036464	cellular_component	cytoplasmic ribonucleoprotein granule
H	0043484	biological_process	regulation of RNA splicing
H	0045893	biological_process	positive regulation of DNA-templated transcription
H	1904672	biological_process	regulation of somatic stem cell population maintenance
I	0000124	cellular_component	SAGA complex
I	0003713	molecular_function	transcription coactivator activity
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0006282	biological_process	regulation of DNA repair
I	0043484	biological_process	regulation of RNA splicing
I	0045893	biological_process	positive regulation of DNA-templated transcription
I	0046982	molecular_function	protein heterodimerization activity
I	0051457	biological_process	maintenance of protein location in nucleus
K	0000118	cellular_component	histone deacetylase complex
K	0000124	cellular_component	SAGA complex
K	0003677	molecular_function	DNA binding
K	0003713	molecular_function	transcription coactivator activity
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005669	cellular_component	transcription factor TFIID complex
K	0006282	biological_process	regulation of DNA repair
K	0006338	biological_process	chromatin remodeling
K	0006355	biological_process	regulation of DNA-templated transcription
K	0006357	biological_process	regulation of transcription by RNA polymerase II
K	0006367	biological_process	transcription initiation at RNA polymerase II promoter
K	0016251	molecular_function	RNA polymerase II general transcription initiation factor activity
K	0043484	biological_process	regulation of RNA splicing
K	0045893	biological_process	positive regulation of DNA-templated transcription
K	0046695	cellular_component	SLIK (SAGA-like) complex
K	0046982	molecular_function	protein heterodimerization activity
K	0051123	biological_process	RNA polymerase II preinitiation complex assembly
K	0070062	cellular_component	extracellular exosome
K	1904672	biological_process	regulation of somatic stem cell population maintenance
L	0000124	cellular_component	SAGA complex
L	0000226	biological_process	microtubule cytoskeleton organization
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005654	cellular_component	nucleoplasm
L	0005730	cellular_component	nucleolus
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0005856	cellular_component	cytoskeleton
L	0006282	biological_process	regulation of DNA repair
L	0006357	biological_process	regulation of transcription by RNA polymerase II
L	0006997	biological_process	nucleus organization
L	0007026	biological_process	negative regulation of microtubule depolymerization
L	0007601	biological_process	visual perception
L	0015630	cellular_component	microtubule cytoskeleton
L	0016363	cellular_component	nuclear matrix
L	0033276	cellular_component	transcription factor TFTC complex
L	0043484	biological_process	regulation of RNA splicing
L	0045893	biological_process	positive regulation of DNA-templated transcription
M	0000124	cellular_component	SAGA complex
M	0000492	biological_process	box C/D snoRNP assembly
M	0000976	molecular_function	transcription cis-regulatory region binding
M	0002039	molecular_function	p53 binding
M	0003677	molecular_function	DNA binding
M	0003713	molecular_function	transcription coactivator activity
M	0004402	molecular_function	histone acetyltransferase activity
M	0005515	molecular_function	protein binding
M	0005634	cellular_component	nucleus
M	0005654	cellular_component	nucleoplasm
M	0005669	cellular_component	transcription factor TFIID complex
M	0006282	biological_process	regulation of DNA repair
M	0006338	biological_process	chromatin remodeling
M	0006352	biological_process	DNA-templated transcription initiation
M	0006355	biological_process	regulation of DNA-templated transcription
M	0006357	biological_process	regulation of transcription by RNA polymerase II
M	0006974	biological_process	DNA damage response
M	0016251	molecular_function	RNA polymerase II general transcription initiation factor activity
M	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
M	0033276	cellular_component	transcription factor TFTC complex
M	0042789	biological_process	mRNA transcription by RNA polymerase II
M	0043066	biological_process	negative regulation of apoptotic process
M	0043484	biological_process	regulation of RNA splicing
M	0045893	biological_process	positive regulation of DNA-templated transcription
M	0045944	biological_process	positive regulation of transcription by RNA polymerase II
M	0046982	molecular_function	protein heterodimerization activity
M	0050821	biological_process	protein stabilization
M	0051117	molecular_function	ATPase binding
M	0051123	biological_process	RNA polymerase II preinitiation complex assembly
M	0060261	biological_process	positive regulation of transcription initiation by RNA polymerase II
M	0060760	biological_process	positive regulation of response to cytokine stimulus
M	0070555	biological_process	response to interleukin-1
M	0070742	molecular_function	C2H2 zinc finger domain binding
M	0070761	cellular_component	pre-snoRNP complex
M	0071339	cellular_component	MLL1 complex
M	0140297	molecular_function	DNA-binding transcription factor binding
M	1902065	biological_process	response to L-glutamate
M	1902166	biological_process	negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
O	0000082	biological_process	G1/S transition of mitotic cell cycle
O	0000124	cellular_component	SAGA complex
O	0001673	cellular_component	male germ cell nucleus
O	0001701	biological_process	in utero embryonic development
O	0001756	biological_process	somitogenesis
O	0001889	biological_process	liver development
O	0001892	biological_process	embryonic placenta development
O	0003677	molecular_function	DNA binding
O	0004402	molecular_function	histone acetyltransferase activity
O	0005515	molecular_function	protein binding
O	0005634	cellular_component	nucleus
O	0005654	cellular_component	nucleoplasm
O	0005669	cellular_component	transcription factor TFIID complex
O	0005737	cellular_component	cytoplasm
O	0006282	biological_process	regulation of DNA repair
O	0006338	biological_process	chromatin remodeling
O	0006352	biological_process	DNA-templated transcription initiation
O	0006355	biological_process	regulation of DNA-templated transcription
O	0006357	biological_process	regulation of transcription by RNA polymerase II
O	0006366	biological_process	transcription by RNA polymerase II
O	0006367	biological_process	transcription initiation at RNA polymerase II promoter
O	0006915	biological_process	apoptotic process
O	0010467	biological_process	gene expression
O	0010468	biological_process	regulation of gene expression
O	0016251	molecular_function	RNA polymerase II general transcription initiation factor activity
O	0019899	molecular_function	enzyme binding
O	0030331	molecular_function	nuclear estrogen receptor binding
O	0033276	cellular_component	transcription factor TFTC complex
O	0035264	biological_process	multicellular organism growth
O	0036285	biological_process	SAGA complex assembly
O	0042789	biological_process	mRNA transcription by RNA polymerase II
O	0042802	molecular_function	identical protein binding
O	0043229	cellular_component	intracellular organelle
O	0043484	biological_process	regulation of RNA splicing
O	0045893	biological_process	positive regulation of DNA-templated transcription
O	0048371	biological_process	lateral mesodermal cell differentiation
O	0048471	cellular_component	perinuclear region of cytoplasm
O	0051123	biological_process	RNA polymerase II preinitiation complex assembly
O	0060173	biological_process	limb development
O	0060261	biological_process	positive regulation of transcription initiation by RNA polymerase II
O	0065003	biological_process	protein-containing complex assembly
O	0070063	molecular_function	RNA polymerase binding
O	0070365	biological_process	hepatocyte differentiation
O	0097550	cellular_component	transcription preinitiation complex
O	1905069	biological_process	allantois development
O	1990841	molecular_function	promoter-specific chromatin binding
R	0000124	cellular_component	SAGA complex
R	0003677	molecular_function	DNA binding
R	0003713	molecular_function	transcription coactivator activity
R	0005515	molecular_function	protein binding
R	0005634	cellular_component	nucleus
R	0005654	cellular_component	nucleoplasm
R	0005669	cellular_component	transcription factor TFIID complex
R	0006282	biological_process	regulation of DNA repair
R	0006352	biological_process	DNA-templated transcription initiation
R	0006355	biological_process	regulation of DNA-templated transcription
R	0006357	biological_process	regulation of transcription by RNA polymerase II
R	0006366	biological_process	transcription by RNA polymerase II
R	0006367	biological_process	transcription initiation at RNA polymerase II promoter
R	0016251	molecular_function	RNA polymerase II general transcription initiation factor activity
R	0017025	molecular_function	TBP-class protein binding
R	0033276	cellular_component	transcription factor TFTC complex
R	0042789	biological_process	mRNA transcription by RNA polymerase II
R	0043484	biological_process	regulation of RNA splicing
R	0045893	biological_process	positive regulation of DNA-templated transcription
R	0045944	biological_process	positive regulation of transcription by RNA polymerase II
R	0046982	molecular_function	protein heterodimerization activity
R	0051123	biological_process	RNA polymerase II preinitiation complex assembly
R	0060261	biological_process	positive regulation of transcription initiation by RNA polymerase II
R	0140297	molecular_function	DNA-binding transcription factor binding

Functional Information from PROSITE/UniProt

site_id	PS00196
Number of Residues	15
Details	COPPER_BLUE Type-1 copper (blue) proteins signature. GeiSpFLCs.GsHqv..Q

Chain	Residue	Details
C	GLY2650-GLN2664

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site_id	PS00290
Number of Residues	7
Details	IG_MHC Immunoglobulins and major histocompatibility complex proteins signature. FVCSATH

Chain	Residue	Details
A	PHE287-HIS293

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site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. LASAgeDqRLKLWDL

Chain	Residue	Details
H	LEU483-LEU497
H	ILE525-ILE539

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
K	SER495
K	SER501
A	GLU352
A	ARG429
A	ASN916

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site_id	SWS_FT_FI2
Number of Residues	3
Details	MOD_RES: Asymmetric dimethylarginine => ECO:0007744|PubMed:24129315

Chain	Residue	Details
K	ARG555
K	ARG561
K	ARG593

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site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:24275569

Chain	Residue	Details
I	SER334

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site_id	SWS_FT_FI4
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25218447, ECO:0007744|PubMed:25755297, ECO:0007744|PubMed:28112733

Chain	Residue	Details
I	LYS271
M	SER196

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site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q8VI33

Chain	Residue	Details
M	SER155

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site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:19690332

Chain	Residue	Details
M	SER158

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site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:19690332

Chain	Residue	Details
M	THR159

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site_id	SWS_FT_FI8
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:18669648

Chain	Residue	Details
M	THR161

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site_id	SWS_FT_FI9
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:Q8VI33

Chain	Residue	Details
M	THR164

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site_id	SWS_FT_FI10
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163

Chain	Residue	Details
M	THR178

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site_id	SWS_FT_FI11
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648

Chain	Residue	Details
M	SER181

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