7UIG

Mediator-PIC Early (Mediator A)

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
a	0000122	biological_process	negative regulation of transcription by RNA polymerase II
a	0003674	molecular_function	molecular_function
a	0003712	molecular_function	transcription coregulator activity
a	0005515	molecular_function	protein binding
a	0005634	cellular_component	nucleus
a	0006357	biological_process	regulation of transcription by RNA polymerase II
a	0016592	cellular_component	mediator complex
a	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
a	0045944	biological_process	positive regulation of transcription by RNA polymerase II
a	0051123	biological_process	RNA polymerase II preinitiation complex assembly
a	0060261	biological_process	positive regulation of transcription initiation by RNA polymerase II
a	0070847	cellular_component	core mediator complex
d	0000979	molecular_function	RNA polymerase II core promoter sequence-specific DNA binding
d	0003712	molecular_function	transcription coregulator activity
d	0005515	molecular_function	protein binding
d	0005634	cellular_component	nucleus
d	0006357	biological_process	regulation of transcription by RNA polymerase II
d	0006366	biological_process	transcription by RNA polymerase II
d	0016592	cellular_component	mediator complex
d	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
d	0034605	biological_process	cellular response to heat
d	0051123	biological_process	RNA polymerase II preinitiation complex assembly
d	0060261	biological_process	positive regulation of transcription initiation by RNA polymerase II
d	0070847	cellular_component	core mediator complex
f	0003712	molecular_function	transcription coregulator activity
f	0003713	molecular_function	transcription coactivator activity
f	0005515	molecular_function	protein binding
f	0005634	cellular_component	nucleus
f	0006357	biological_process	regulation of transcription by RNA polymerase II
f	0016592	cellular_component	mediator complex
f	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
f	0045944	biological_process	positive regulation of transcription by RNA polymerase II
f	0051123	biological_process	RNA polymerase II preinitiation complex assembly
f	0060261	biological_process	positive regulation of transcription initiation by RNA polymerase II
f	0070847	cellular_component	core mediator complex
g	0000122	biological_process	negative regulation of transcription by RNA polymerase II
g	0003712	molecular_function	transcription coregulator activity
g	0003713	molecular_function	transcription coactivator activity
g	0005515	molecular_function	protein binding
g	0005634	cellular_component	nucleus
g	0006357	biological_process	regulation of transcription by RNA polymerase II
g	0016592	cellular_component	mediator complex
g	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
g	0045944	biological_process	positive regulation of transcription by RNA polymerase II
g	0051123	biological_process	RNA polymerase II preinitiation complex assembly
g	0060261	biological_process	positive regulation of transcription initiation by RNA polymerase II
g	0070847	cellular_component	core mediator complex
h	0000122	biological_process	negative regulation of transcription by RNA polymerase II
h	0000978	molecular_function	RNA polymerase II cis-regulatory region sequence-specific DNA binding
h	0003677	molecular_function	DNA binding
h	0003712	molecular_function	transcription coregulator activity
h	0003714	molecular_function	transcription corepressor activity
h	0005515	molecular_function	protein binding
h	0005634	cellular_component	nucleus
h	0006357	biological_process	regulation of transcription by RNA polymerase II
h	0016592	cellular_component	mediator complex
h	0017025	molecular_function	TBP-class protein binding
h	0030674	molecular_function	protein-macromolecule adaptor activity
h	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
h	0045944	biological_process	positive regulation of transcription by RNA polymerase II
h	0051123	biological_process	RNA polymerase II preinitiation complex assembly
h	0060261	biological_process	positive regulation of transcription initiation by RNA polymerase II
h	0070847	cellular_component	core mediator complex
i	0000122	biological_process	negative regulation of transcription by RNA polymerase II
i	0003712	molecular_function	transcription coregulator activity
i	0003713	molecular_function	transcription coactivator activity
i	0005198	molecular_function	structural molecule activity
i	0005634	cellular_component	nucleus
i	0005829	cellular_component	cytosol
i	0006357	biological_process	regulation of transcription by RNA polymerase II
i	0016592	cellular_component	mediator complex
i	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
i	0045944	biological_process	positive regulation of transcription by RNA polymerase II
i	0051123	biological_process	RNA polymerase II preinitiation complex assembly
i	0060261	biological_process	positive regulation of transcription initiation by RNA polymerase II
i	0070847	cellular_component	core mediator complex
j	0000122	biological_process	negative regulation of transcription by RNA polymerase II
j	0003674	molecular_function	molecular_function
j	0003712	molecular_function	transcription coregulator activity
j	0005515	molecular_function	protein binding
j	0005634	cellular_component	nucleus
j	0006357	biological_process	regulation of transcription by RNA polymerase II
j	0016592	cellular_component	mediator complex
j	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
j	0045944	biological_process	positive regulation of transcription by RNA polymerase II
j	0051123	biological_process	RNA polymerase II preinitiation complex assembly
j	0060261	biological_process	positive regulation of transcription initiation by RNA polymerase II
j	0070847	cellular_component	core mediator complex
k	0000122	biological_process	negative regulation of transcription by RNA polymerase II
k	0001097	molecular_function	TFIIH-class transcription factor complex binding
k	0003712	molecular_function	transcription coregulator activity
k	0003713	molecular_function	transcription coactivator activity
k	0005515	molecular_function	protein binding
k	0005634	cellular_component	nucleus
k	0006357	biological_process	regulation of transcription by RNA polymerase II
k	0016592	cellular_component	mediator complex
k	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
k	0045944	biological_process	positive regulation of transcription by RNA polymerase II
k	0051123	biological_process	RNA polymerase II preinitiation complex assembly
k	0060261	biological_process	positive regulation of transcription initiation by RNA polymerase II
k	0070847	cellular_component	core mediator complex
n	0000122	biological_process	negative regulation of transcription by RNA polymerase II
n	0001093	molecular_function	TFIIB-class transcription factor binding
n	0003712	molecular_function	transcription coregulator activity
n	0005515	molecular_function	protein binding
n	0005634	cellular_component	nucleus
n	0006357	biological_process	regulation of transcription by RNA polymerase II
n	0016592	cellular_component	mediator complex
n	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
n	0045944	biological_process	positive regulation of transcription by RNA polymerase II
n	0051123	biological_process	RNA polymerase II preinitiation complex assembly
n	0060261	biological_process	positive regulation of transcription initiation by RNA polymerase II
n	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
n	0070847	cellular_component	core mediator complex
q	0000979	molecular_function	RNA polymerase II core promoter sequence-specific DNA binding
q	0001139	molecular_function	RNA polymerase II complex recruiting activity
q	0003712	molecular_function	transcription coregulator activity
q	0005515	molecular_function	protein binding
q	0005634	cellular_component	nucleus
q	0006357	biological_process	regulation of transcription by RNA polymerase II
q	0006367	biological_process	transcription initiation at RNA polymerase II promoter
q	0016592	cellular_component	mediator complex
q	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
q	0034605	biological_process	cellular response to heat
q	0045944	biological_process	positive regulation of transcription by RNA polymerase II
q	0051123	biological_process	RNA polymerase II preinitiation complex assembly
q	0060261	biological_process	positive regulation of transcription initiation by RNA polymerase II
q	0070847	cellular_component	core mediator complex
q	0140297	molecular_function	DNA-binding transcription factor binding
r	0000979	molecular_function	RNA polymerase II core promoter sequence-specific DNA binding
r	0001113	biological_process	transcription open complex formation at RNA polymerase II promoter
r	0003712	molecular_function	transcription coregulator activity
r	0003713	molecular_function	transcription coactivator activity
r	0005515	molecular_function	protein binding
r	0005634	cellular_component	nucleus
r	0006357	biological_process	regulation of transcription by RNA polymerase II
r	0006369	biological_process	termination of RNA polymerase II transcription
r	0016592	cellular_component	mediator complex
r	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
r	0034605	biological_process	cellular response to heat
r	0045944	biological_process	positive regulation of transcription by RNA polymerase II
r	0051123	biological_process	RNA polymerase II preinitiation complex assembly
r	0060261	biological_process	positive regulation of transcription initiation by RNA polymerase II
r	0070847	cellular_component	core mediator complex
s	0000122	biological_process	negative regulation of transcription by RNA polymerase II
s	0001113	biological_process	transcription open complex formation at RNA polymerase II promoter
s	0003712	molecular_function	transcription coregulator activity
s	0003713	molecular_function	transcription coactivator activity
s	0005634	cellular_component	nucleus
s	0006357	biological_process	regulation of transcription by RNA polymerase II
s	0016592	cellular_component	mediator complex
s	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
s	0045944	biological_process	positive regulation of transcription by RNA polymerase II
s	0051123	biological_process	RNA polymerase II preinitiation complex assembly
s	0060261	biological_process	positive regulation of transcription initiation by RNA polymerase II
s	0070847	cellular_component	core mediator complex
t	0001094	molecular_function	TFIID-class transcription factor complex binding
t	0003712	molecular_function	transcription coregulator activity
t	0003713	molecular_function	transcription coactivator activity
t	0005515	molecular_function	protein binding
t	0005634	cellular_component	nucleus
t	0006357	biological_process	regulation of transcription by RNA polymerase II
t	0010688	biological_process	negative regulation of ribosomal protein gene transcription by RNA polymerase II
t	0016592	cellular_component	mediator complex
t	0019904	molecular_function	protein domain specific binding
t	0031669	biological_process	cellular response to nutrient levels
t	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
t	0032991	cellular_component	protein-containing complex
t	0045944	biological_process	positive regulation of transcription by RNA polymerase II
t	0051123	biological_process	RNA polymerase II preinitiation complex assembly
t	0060261	biological_process	positive regulation of transcription initiation by RNA polymerase II
t	0070847	cellular_component	core mediator complex
u	0000122	biological_process	negative regulation of transcription by RNA polymerase II
u	0003712	molecular_function	transcription coregulator activity
u	0003713	molecular_function	transcription coactivator activity
u	0003714	molecular_function	transcription corepressor activity
u	0005515	molecular_function	protein binding
u	0005634	cellular_component	nucleus
u	0006357	biological_process	regulation of transcription by RNA polymerase II
u	0016592	cellular_component	mediator complex
u	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
u	0045944	biological_process	positive regulation of transcription by RNA polymerase II
u	0051123	biological_process	RNA polymerase II preinitiation complex assembly
u	0060261	biological_process	positive regulation of transcription initiation by RNA polymerase II
u	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
u	0070847	cellular_component	core mediator complex
v	0003674	molecular_function	molecular_function
v	0003712	molecular_function	transcription coregulator activity
v	0005515	molecular_function	protein binding
v	0005634	cellular_component	nucleus
v	0006357	biological_process	regulation of transcription by RNA polymerase II
v	0016592	cellular_component	mediator complex
v	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
v	0045944	biological_process	positive regulation of transcription by RNA polymerase II
v	0051123	biological_process	RNA polymerase II preinitiation complex assembly
v	0060261	biological_process	positive regulation of transcription initiation by RNA polymerase II
v	0070847	cellular_component	core mediator complex
w	0003712	molecular_function	transcription coregulator activity
w	0003713	molecular_function	transcription coactivator activity
w	0005515	molecular_function	protein binding
w	0005634	cellular_component	nucleus
w	0006281	biological_process	DNA repair
w	0006310	biological_process	DNA recombination
w	0006311	biological_process	meiotic gene conversion
w	0006355	biological_process	regulation of DNA-templated transcription
w	0006357	biological_process	regulation of transcription by RNA polymerase II
w	0006366	biological_process	transcription by RNA polymerase II
w	0016592	cellular_component	mediator complex
w	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
w	0051123	biological_process	RNA polymerase II preinitiation complex assembly
w	0060261	biological_process	positive regulation of transcription initiation by RNA polymerase II
w	0070847	cellular_component	core mediator complex
z	0000428	cellular_component	DNA-directed RNA polymerase complex
z	0001055	molecular_function	RNA polymerase II activity
z	0001172	biological_process	RNA-templated transcription
z	0003677	molecular_function	DNA binding
z	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
z	0003968	molecular_function	RNA-dependent RNA polymerase activity
z	0005515	molecular_function	protein binding
z	0005634	cellular_component	nucleus
z	0005665	cellular_component	RNA polymerase II, core complex
z	0005739	cellular_component	mitochondrion
z	0006351	biological_process	DNA-templated transcription
z	0006366	biological_process	transcription by RNA polymerase II
z	0006367	biological_process	transcription initiation at RNA polymerase II promoter
z	0006368	biological_process	transcription elongation by RNA polymerase II
z	0008270	molecular_function	zinc ion binding
z	0010494	cellular_component	cytoplasmic stress granule
z	0016779	molecular_function	nucleotidyltransferase activity
z	0019985	biological_process	translesion synthesis
z	0034062	molecular_function	5'-3' RNA polymerase activity
z	0046872	molecular_function	metal ion binding

Functional Information from PROSITE/UniProt

site_id	PS00115
Number of Residues	7
Details	RNA_POL_II_REPEAT Eukaryotic RNA polymerase II heptapeptide repeat. YSPTSPA

Chain	Residue	Details
z	TYR-11-ALA-5
z	TYR52-SER58
z	TYR59-SER65
z	TYR66-SER72
z	TYR73-SER79
z	TYR80-SER86
z	TYR87-SER93
z	TYR94-ALA100
z	TYR101-SER107
z	TYR108-SER114
z	TYR115-SER121
z	TYR-4-SER2
z	TYR122-SER128
z	TYR129-ASN135
z	TYR136-SER142
z	TYR3-SER9
z	TYR10-SER16
z	TYR17-SER23
z	TYR24-SER30
z	TYR31-SER37
z	TYR38-SER44
z	TYR45-SER51

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	11
Details	BINDING: BINDING => ECO:0000269|PubMed:23151482, ECO:0000269|PubMed:25652824, ECO:0007744|PDB:4BBR, ECO:0007744|PDB:4V1M

Chain	Residue	Details
z	CYS-1493
z	ASP-1077
z	ASP-1075
z	CYS-1490
z	CYS-1483
z	HIS-1480
z	CYS-1453
z	CYS-1450
z	CYS-1412
z	CYS-1393
z	ASP-1079

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site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:18407956

Chain	Residue	Details
z	THR-89

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site_id	SWS_FT_FI3
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047

Chain	Residue	Details
z	LYS-865
z	LYS-210

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site_id	SWS_FT_FI4
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:32142654, ECO:0007744|PubMed:22106047

Chain	Residue	Details
z	LYS-314

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	4
Details	M-CSA 788

Chain	Residue	Details
z	ASP-1079	metal ligand
z	ASP-1077	metal ligand
z	ASP-1075	metal ligand
z	HIS-475	proton acceptor, proton donor

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