7QQ3

Cryo-EM structure of the E.coli 50S ribosomal subunit in complex with the antibiotic Myxovalargin A.

Functional Information from GO Data

Chain	GOid	namespace	contents
K	0000027	biological_process	ribosomal large subunit assembly
K	0002181	biological_process	cytoplasmic translation
K	0003723	molecular_function	RNA binding
K	0003735	molecular_function	structural constituent of ribosome
K	0005515	molecular_function	protein binding
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0005840	cellular_component	ribosome
K	0006412	biological_process	translation
K	0008270	molecular_function	zinc ion binding
K	0015934	cellular_component	large ribosomal subunit
K	0016740	molecular_function	transferase activity
K	0019843	molecular_function	rRNA binding
K	0022625	cellular_component	cytosolic large ribosomal subunit
K	0032297	biological_process	negative regulation of DNA-templated DNA replication initiation
K	1990082	cellular_component	DnaA-L2 complex
K	1990904	cellular_component	ribonucleoprotein complex
L	0000027	biological_process	ribosomal large subunit assembly
L	0002181	biological_process	cytoplasmic translation
L	0003735	molecular_function	structural constituent of ribosome
L	0005515	molecular_function	protein binding
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0005840	cellular_component	ribosome
L	0006412	biological_process	translation
L	0019843	molecular_function	rRNA binding
L	0022625	cellular_component	cytosolic large ribosomal subunit
L	1990904	cellular_component	ribonucleoprotein complex
M	0001070	molecular_function	RNA-binding transcription regulator activity
M	0002181	biological_process	cytoplasmic translation
M	0003677	molecular_function	DNA binding
M	0003735	molecular_function	structural constituent of ribosome
M	0005515	molecular_function	protein binding
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0005840	cellular_component	ribosome
M	0006353	biological_process	DNA-templated transcription termination
M	0006412	biological_process	translation
M	0006417	biological_process	regulation of translation
M	0015934	cellular_component	large ribosomal subunit
M	0017148	biological_process	negative regulation of translation
M	0019843	molecular_function	rRNA binding
M	0022625	cellular_component	cytosolic large ribosomal subunit
M	0030371	molecular_function	translation repressor activity
M	0031555	biological_process	transcriptional attenuation
M	0042255	biological_process	ribosome assembly
M	0045892	biological_process	negative regulation of DNA-templated transcription
M	0046677	biological_process	response to antibiotic
M	0048027	molecular_function	mRNA 5'-UTR binding
M	0060698	molecular_function	endoribonuclease inhibitor activity
M	1990904	cellular_component	ribonucleoprotein complex
M	2000766	biological_process	negative regulation of cytoplasmic translation
O	0000027	biological_process	ribosomal large subunit assembly
O	0002181	biological_process	cytoplasmic translation
O	0003735	molecular_function	structural constituent of ribosome
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0005840	cellular_component	ribosome
O	0006412	biological_process	translation
O	0019843	molecular_function	rRNA binding
O	0022625	cellular_component	cytosolic large ribosomal subunit
O	0046677	biological_process	response to antibiotic
O	0070180	molecular_function	large ribosomal subunit rRNA binding
O	1990904	cellular_component	ribonucleoprotein complex
R	0002181	biological_process	cytoplasmic translation
R	0003729	molecular_function	mRNA binding
R	0003735	molecular_function	structural constituent of ribosome
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0005840	cellular_component	ribosome
R	0006412	biological_process	translation
R	0008270	molecular_function	zinc ion binding
R	0017148	biological_process	negative regulation of translation
R	0022625	cellular_component	cytosolic large ribosomal subunit
R	0043229	cellular_component	intracellular organelle
R	0048027	molecular_function	mRNA 5'-UTR binding
R	0070180	molecular_function	large ribosomal subunit rRNA binding
R	1990904	cellular_component	ribonucleoprotein complex
S	0002181	biological_process	cytoplasmic translation
S	0003735	molecular_function	structural constituent of ribosome
S	0005515	molecular_function	protein binding
S	0005737	cellular_component	cytoplasm
S	0005829	cellular_component	cytosol
S	0005840	cellular_component	ribosome
S	0006412	biological_process	translation
S	0015934	cellular_component	large ribosomal subunit
S	0019843	molecular_function	rRNA binding
S	0022625	cellular_component	cytosolic large ribosomal subunit
S	0070180	molecular_function	large ribosomal subunit rRNA binding
S	1990904	cellular_component	ribonucleoprotein complex
T	0002181	biological_process	cytoplasmic translation
T	0003735	molecular_function	structural constituent of ribosome
T	0005515	molecular_function	protein binding
T	0005737	cellular_component	cytoplasm
T	0005840	cellular_component	ribosome
T	0006412	biological_process	translation
T	0015934	cellular_component	large ribosomal subunit
T	0019843	molecular_function	rRNA binding
T	0022625	cellular_component	cytosolic large ribosomal subunit
T	1990904	cellular_component	ribonucleoprotein complex
U	0000027	biological_process	ribosomal large subunit assembly
U	0000049	molecular_function	tRNA binding
U	0002181	biological_process	cytoplasmic translation
U	0003735	molecular_function	structural constituent of ribosome
U	0005737	cellular_component	cytoplasm
U	0005840	cellular_component	ribosome
U	0006412	biological_process	translation
U	0019843	molecular_function	rRNA binding
U	0022625	cellular_component	cytosolic large ribosomal subunit
U	1990904	cellular_component	ribonucleoprotein complex
V	0000027	biological_process	ribosomal large subunit assembly
V	0002181	biological_process	cytoplasmic translation
V	0003735	molecular_function	structural constituent of ribosome
V	0005515	molecular_function	protein binding
V	0005737	cellular_component	cytoplasm
V	0005829	cellular_component	cytosol
V	0005840	cellular_component	ribosome
V	0006412	biological_process	translation
V	0022625	cellular_component	cytosolic large ribosomal subunit
V	1990904	cellular_component	ribonucleoprotein complex
W	0002181	biological_process	cytoplasmic translation
W	0003735	molecular_function	structural constituent of ribosome
W	0005737	cellular_component	cytoplasm
W	0005829	cellular_component	cytosol
W	0005840	cellular_component	ribosome
W	0006412	biological_process	translation
W	0008097	molecular_function	5S rRNA binding
W	0019843	molecular_function	rRNA binding
W	0022625	cellular_component	cytosolic large ribosomal subunit
W	0043229	cellular_component	intracellular organelle
W	1990904	cellular_component	ribonucleoprotein complex
X	0000027	biological_process	ribosomal large subunit assembly
X	0002181	biological_process	cytoplasmic translation
X	0003735	molecular_function	structural constituent of ribosome
X	0005737	cellular_component	cytoplasm
X	0005829	cellular_component	cytosol
X	0005840	cellular_component	ribosome
X	0006412	biological_process	translation
X	0019843	molecular_function	rRNA binding
X	0022625	cellular_component	cytosolic large ribosomal subunit
X	0070180	molecular_function	large ribosomal subunit rRNA binding
X	1990904	cellular_component	ribonucleoprotein complex
Y	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
Y	0002181	biological_process	cytoplasmic translation
Y	0003729	molecular_function	mRNA binding
Y	0003735	molecular_function	structural constituent of ribosome
Y	0005737	cellular_component	cytoplasm
Y	0005829	cellular_component	cytosol
Y	0005840	cellular_component	ribosome
Y	0006412	biological_process	translation
Y	0017148	biological_process	negative regulation of translation
Y	0019843	molecular_function	rRNA binding
Y	0022625	cellular_component	cytosolic large ribosomal subunit
Y	0070180	molecular_function	large ribosomal subunit rRNA binding
Y	1990904	cellular_component	ribonucleoprotein complex
Z	0002181	biological_process	cytoplasmic translation
Z	0003723	molecular_function	RNA binding
Z	0003735	molecular_function	structural constituent of ribosome
Z	0005737	cellular_component	cytoplasm
Z	0005829	cellular_component	cytosol
Z	0005840	cellular_component	ribosome
Z	0006412	biological_process	translation
Z	0019843	molecular_function	rRNA binding
Z	0022625	cellular_component	cytosolic large ribosomal subunit
Z	0043229	cellular_component	intracellular organelle
Z	1990904	cellular_component	ribonucleoprotein complex
a	0002181	biological_process	cytoplasmic translation
a	0003735	molecular_function	structural constituent of ribosome
a	0005515	molecular_function	protein binding
a	0005737	cellular_component	cytoplasm
a	0005829	cellular_component	cytosol
a	0005840	cellular_component	ribosome
a	0006412	biological_process	translation
a	0015934	cellular_component	large ribosomal subunit
a	0019843	molecular_function	rRNA binding
a	0022625	cellular_component	cytosolic large ribosomal subunit
a	0042255	biological_process	ribosome assembly
a	0042256	biological_process	cytosolic ribosome assembly
a	0046677	biological_process	response to antibiotic
a	0070180	molecular_function	large ribosomal subunit rRNA binding
a	1902626	biological_process	assembly of large subunit precursor of preribosome
a	1990904	cellular_component	ribonucleoprotein complex
b	0000027	biological_process	ribosomal large subunit assembly
b	0002181	biological_process	cytoplasmic translation
b	0003735	molecular_function	structural constituent of ribosome
b	0005515	molecular_function	protein binding
b	0005737	cellular_component	cytoplasm
b	0005840	cellular_component	ribosome
b	0006412	biological_process	translation
b	0019843	molecular_function	rRNA binding
b	0022625	cellular_component	cytosolic large ribosomal subunit
b	1990904	cellular_component	ribonucleoprotein complex
c	0000027	biological_process	ribosomal large subunit assembly
c	0002181	biological_process	cytoplasmic translation
c	0003723	molecular_function	RNA binding
c	0003735	molecular_function	structural constituent of ribosome
c	0005515	molecular_function	protein binding
c	0005737	cellular_component	cytoplasm
c	0005829	cellular_component	cytosol
c	0005840	cellular_component	ribosome
c	0006412	biological_process	translation
c	0019843	molecular_function	rRNA binding
c	0022625	cellular_component	cytosolic large ribosomal subunit
c	0070180	molecular_function	large ribosomal subunit rRNA binding
c	1990904	cellular_component	ribonucleoprotein complex
d	0000027	biological_process	ribosomal large subunit assembly
d	0002181	biological_process	cytoplasmic translation
d	0003723	molecular_function	RNA binding
d	0003735	molecular_function	structural constituent of ribosome
d	0005737	cellular_component	cytoplasm
d	0005829	cellular_component	cytosol
d	0005840	cellular_component	ribosome
d	0006412	biological_process	translation
d	0008097	molecular_function	5S rRNA binding
d	0009314	biological_process	response to radiation
d	0017148	biological_process	negative regulation of translation
d	0019843	molecular_function	rRNA binding
d	0022625	cellular_component	cytosolic large ribosomal subunit
d	1990904	cellular_component	ribonucleoprotein complex
e	0000027	biological_process	ribosomal large subunit assembly
e	0000049	molecular_function	tRNA binding
e	0001558	biological_process	regulation of cell growth
e	0002181	biological_process	cytoplasmic translation
e	0003735	molecular_function	structural constituent of ribosome
e	0005515	molecular_function	protein binding
e	0005737	cellular_component	cytoplasm
e	0005840	cellular_component	ribosome
e	0006412	biological_process	translation
e	0019843	molecular_function	rRNA binding
e	0022625	cellular_component	cytosolic large ribosomal subunit
e	0042256	biological_process	cytosolic ribosome assembly
e	0043022	molecular_function	ribosome binding
e	0090070	biological_process	positive regulation of ribosome biogenesis
e	1902626	biological_process	assembly of large subunit precursor of preribosome
e	1990904	cellular_component	ribonucleoprotein complex
f	0000027	biological_process	ribosomal large subunit assembly
f	0002181	biological_process	cytoplasmic translation
f	0003735	molecular_function	structural constituent of ribosome
f	0005515	molecular_function	protein binding
f	0005737	cellular_component	cytoplasm
f	0005829	cellular_component	cytosol
f	0005840	cellular_component	ribosome
f	0006412	biological_process	translation
f	0019843	molecular_function	rRNA binding
f	0022625	cellular_component	cytosolic large ribosomal subunit
f	1990904	cellular_component	ribonucleoprotein complex
g	0000027	biological_process	ribosomal large subunit assembly
g	0002181	biological_process	cytoplasmic translation
g	0003735	molecular_function	structural constituent of ribosome
g	0005737	cellular_component	cytoplasm
g	0005840	cellular_component	ribosome
g	0006412	biological_process	translation
g	0019843	molecular_function	rRNA binding
g	0022625	cellular_component	cytosolic large ribosomal subunit
g	1990904	cellular_component	ribonucleoprotein complex
h	0000027	biological_process	ribosomal large subunit assembly
h	0002181	biological_process	cytoplasmic translation
h	0003735	molecular_function	structural constituent of ribosome
h	0005737	cellular_component	cytoplasm
h	0005840	cellular_component	ribosome
h	0006412	biological_process	translation
h	0015934	cellular_component	large ribosomal subunit
h	0022625	cellular_component	cytosolic large ribosomal subunit
h	1990904	cellular_component	ribonucleoprotein complex
i	0000027	biological_process	ribosomal large subunit assembly
i	0000302	biological_process	response to reactive oxygen species
i	0002181	biological_process	cytoplasmic translation
i	0003735	molecular_function	structural constituent of ribosome
i	0005515	molecular_function	protein binding
i	0005737	cellular_component	cytoplasm
i	0005829	cellular_component	cytosol
i	0005840	cellular_component	ribosome
i	0006412	biological_process	translation
i	0009314	biological_process	response to radiation
i	0015934	cellular_component	large ribosomal subunit
i	0022625	cellular_component	cytosolic large ribosomal subunit
i	1990904	cellular_component	ribonucleoprotein complex
j	0000027	biological_process	ribosomal large subunit assembly
j	0000049	molecular_function	tRNA binding
j	0002181	biological_process	cytoplasmic translation
j	0003735	molecular_function	structural constituent of ribosome
j	0005737	cellular_component	cytoplasm
j	0005829	cellular_component	cytosol
j	0005840	cellular_component	ribosome
j	0006412	biological_process	translation
j	0022625	cellular_component	cytosolic large ribosomal subunit
j	0043229	cellular_component	intracellular organelle
j	0046677	biological_process	response to antibiotic
j	1990904	cellular_component	ribonucleoprotein complex
k	0002181	biological_process	cytoplasmic translation
k	0003735	molecular_function	structural constituent of ribosome
k	0005515	molecular_function	protein binding
k	0005737	cellular_component	cytoplasm
k	0005840	cellular_component	ribosome
k	0006412	biological_process	translation
k	0022625	cellular_component	cytosolic large ribosomal subunit
k	1990904	cellular_component	ribonucleoprotein complex
l	0002181	biological_process	cytoplasmic translation
l	0003735	molecular_function	structural constituent of ribosome
l	0005737	cellular_component	cytoplasm
l	0005840	cellular_component	ribosome
l	0006412	biological_process	translation
l	0022625	cellular_component	cytosolic large ribosomal subunit
l	1990904	cellular_component	ribonucleoprotein complex
m	0002181	biological_process	cytoplasmic translation
m	0003735	molecular_function	structural constituent of ribosome
m	0005737	cellular_component	cytoplasm
m	0005840	cellular_component	ribosome
m	0006412	biological_process	translation
m	0022625	cellular_component	cytosolic large ribosomal subunit
m	0043229	cellular_component	intracellular organelle
m	1990904	cellular_component	ribonucleoprotein complex

Functional Information from PROSITE/UniProt

site_id	PS00936
Number of Residues	27
Details	RIBOSOMAL_L35 Ribosomal protein L35 signature. KTVrGAaKRFkktgkggfkhkhanl.RH

Chain	Residue	Details
l	LYS5-HIS31

---

site_id	PS00525
Number of Residues	9
Details	RIBOSOMAL_L6_1 Ribosomal protein L6 signature 1. PEpYKGKGV

Chain	Residue	Details
O	PRO154-VAL162

---

site_id	PS00634
Number of Residues	33
Details	RIBOSOMAL_L30 Ribosomal protein L30 signature. TLlgLgLrRigHtVeredtpairgMInaVsfMV

Chain	Residue	Details
h	THR23-VAL55

---

site_id	PS00474
Number of Residues	24
Details	RIBOSOMAL_L3 Ribosomal protein L3 signature. FadvkkvDvtGtSkGKGfaGtvkR

Chain	Residue	Details
L	PHE101-ARG124

---

site_id	PS00582
Number of Residues	20
Details	RIBOSOMAL_L33 Ribosomal protein L33 signature. YtTtKNkrtkPekLElkKFD

Chain	Residue	Details
j	TYR21-ASP40

---

site_id	PS00937
Number of Residues	17
Details	RIBOSOMAL_L20 Ribosomal protein L20 signature. KrqfRqLWIARINaaaR

Chain	Residue	Details
Y	LYS54-ARG70

---

site_id	PS00049
Number of Residues	27
Details	RIBOSOMAL_L14 Ribosomal protein L14 signature. AVVVrtkkgvrrp.DGsvirFdgNacVL

Chain	Residue	Details
S	ALA60-LEU86

---

site_id	PS00467
Number of Residues	12
Details	RIBOSOMAL_L2 Ribosomal protein L2 signature. PtvRGTAmNPvD

Chain	Residue	Details
K	PRO218-ASP229

---

site_id	PS01015
Number of Residues	16
Details	RIBOSOMAL_L19 Ribosomal protein L19 signature. VkRrGAVR.KAKLYYLR

Chain	Residue	Details
X	VAL86-ARG101

---

site_id	PS00579
Number of Residues	15
Details	RIBOSOMAL_L29 Ribosomal protein L29 signature. QSHLLKqVRRDVARV

Chain	Residue	Details
g	GLN39-VAL53

---

site_id	PS00784
Number of Residues	20
Details	RIBOSOMAL_L34 Ribosomal protein L34 signature. KRTFQpsvlkRnrsh.GFraR

Chain	Residue	Details
k	LYS2-ARG21

---

site_id	PS00831
Number of Residues	15
Details	RIBOSOMAL_L27 Ribosomal protein L27 signature. GsIIvRQRGtkfhaG

Chain	Residue	Details
e	GLY34-GLY48

---

site_id	PS00050
Number of Residues	16
Details	RIBOSOMAL_L23 Ribosomal protein L23 signature. KKAYVTLKegqnldfV

Chain	Residue	Details
b	LYS81-VAL96

---

site_id	PS00430
Number of Residues	66
Details	TONB_DEPENDENT_REC_1 TonB-dependent receptor (TBDR) proteins signature 1. mireerllkvlraphvsekastameksntivlkvakdatkaeikaavqklfevevevv.........................................................NTLVVKGK

Chain	Residue	Details
b	MET1-LYS66

---

site_id	PS01108
Number of Residues	18
Details	RIBOSOMAL_L24 Ribosomal protein L24 signature. DDeViVLtGkdKGkr.GkV

Chain	Residue	Details
c	ASP8-VAL25

---

site_id	PS00783
Number of Residues	23
Details	RIBOSOMAL_L13 Ribosomal protein L13 signature. VKGMLPkgpl.GRamfrkLkVYaG

Chain	Residue	Details
R	VAL105-GLY127

---

site_id	PS00464
Number of Residues	25
Details	RIBOSOMAL_L22 Ribosomal protein L22 signature. KriMpRAkGRadrilkrtSHITVvV

Chain	Residue	Details
a	LYS83-VAL107

---

site_id	PS00586
Number of Residues	12
Details	RIBOSOMAL_L16_1 Ribosomal protein L16 signature 1. KRqGKIWIRVFP

Chain	Residue	Details
U	LYS58-PRO69

---

site_id	PS00701
Number of Residues	12
Details	RIBOSOMAL_L16_2 Ribosomal protein L16 signature 2. RMGkGKGnveyW

Chain	Residue	Details
U	ARG81-TRP92

---

site_id	PS01167
Number of Residues	23
Details	RIBOSOMAL_L17 Ribosomal protein L17 signature. IkTTlpKaKelrrvvEpLITlAK

Chain	Residue	Details
V	ILE34-LYS56

---

site_id	PS00475
Number of Residues	31
Details	RIBOSOMAL_L15 Ribosomal protein L15 signature. KVILaGeVttp..VtVrglrVTkgAraaIeaaG

Chain	Residue	Details
T	LYS109-GLY139

---

site_id	PS00828
Number of Residues	27
Details	RIBOSOMAL_L36 Ribosomal protein L36 signature. CrnCkiVkRdgvIrViCsaepKHkQrQ

Chain	Residue	Details
m	CYS11-GLN37

---

site_id	PS01169
Number of Residues	23
Details	RIBOSOMAL_L21 Ribosomal protein L21 signature. VkivKfrrRKhyrkqqGHRQwfT

Chain	Residue	Details
Z	VAL72-THR94

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000255|HAMAP-Rule:MF_01365, ECO:0000269|PubMed:18723842

Chain	Residue	Details
O	LYS44

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: N5-methylglutamine => ECO:0000269|PubMed:365579, ECO:0000305|PubMed:331083

Chain	Residue	Details
L	GLN150

---