Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7KEL

Dihydrodipicolinate synthase (DHDPS) from C.jejuni, H59K mutant with pyruvate bound in the active site

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0008700	molecular_function	4-hydroxy-2-oxoglutarate aldolase activity
A	0008840	molecular_function	4-hydroxy-tetrahydrodipicolinate synthase activity
A	0009085	biological_process	lysine biosynthetic process
A	0009089	biological_process	lysine biosynthetic process via diaminopimelate
A	0009436	biological_process	glyoxylate catabolic process
A	0016829	molecular_function	lyase activity
A	0019877	biological_process	diaminopimelate biosynthetic process
A	0044281	biological_process	small molecule metabolic process
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0008700	molecular_function	4-hydroxy-2-oxoglutarate aldolase activity
B	0008840	molecular_function	4-hydroxy-tetrahydrodipicolinate synthase activity
B	0009085	biological_process	lysine biosynthetic process
B	0009089	biological_process	lysine biosynthetic process via diaminopimelate
B	0009436	biological_process	glyoxylate catabolic process
B	0016829	molecular_function	lyase activity
B	0019877	biological_process	diaminopimelate biosynthetic process
B	0044281	biological_process	small molecule metabolic process
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0008700	molecular_function	4-hydroxy-2-oxoglutarate aldolase activity
C	0008840	molecular_function	4-hydroxy-tetrahydrodipicolinate synthase activity
C	0009085	biological_process	lysine biosynthetic process
C	0009089	biological_process	lysine biosynthetic process via diaminopimelate
C	0009436	biological_process	glyoxylate catabolic process
C	0016829	molecular_function	lyase activity
C	0019877	biological_process	diaminopimelate biosynthetic process
C	0044281	biological_process	small molecule metabolic process
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0008700	molecular_function	4-hydroxy-2-oxoglutarate aldolase activity
D	0008840	molecular_function	4-hydroxy-tetrahydrodipicolinate synthase activity
D	0009085	biological_process	lysine biosynthetic process
D	0009089	biological_process	lysine biosynthetic process via diaminopimelate
D	0009436	biological_process	glyoxylate catabolic process
D	0016829	molecular_function	lyase activity
D	0019877	biological_process	diaminopimelate biosynthetic process
D	0044281	biological_process	small molecule metabolic process
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0008700	molecular_function	4-hydroxy-2-oxoglutarate aldolase activity
E	0008840	molecular_function	4-hydroxy-tetrahydrodipicolinate synthase activity
E	0009085	biological_process	lysine biosynthetic process
E	0009089	biological_process	lysine biosynthetic process via diaminopimelate
E	0009436	biological_process	glyoxylate catabolic process
E	0016829	molecular_function	lyase activity
E	0019877	biological_process	diaminopimelate biosynthetic process
E	0044281	biological_process	small molecule metabolic process
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0008700	molecular_function	4-hydroxy-2-oxoglutarate aldolase activity
F	0008840	molecular_function	4-hydroxy-tetrahydrodipicolinate synthase activity
F	0009085	biological_process	lysine biosynthetic process
F	0009089	biological_process	lysine biosynthetic process via diaminopimelate
F	0009436	biological_process	glyoxylate catabolic process
F	0016829	molecular_function	lyase activity
F	0019877	biological_process	diaminopimelate biosynthetic process
F	0044281	biological_process	small molecule metabolic process
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0008700	molecular_function	4-hydroxy-2-oxoglutarate aldolase activity
G	0008840	molecular_function	4-hydroxy-tetrahydrodipicolinate synthase activity
G	0009085	biological_process	lysine biosynthetic process
G	0009089	biological_process	lysine biosynthetic process via diaminopimelate
G	0009436	biological_process	glyoxylate catabolic process
G	0016829	molecular_function	lyase activity
G	0019877	biological_process	diaminopimelate biosynthetic process
G	0044281	biological_process	small molecule metabolic process
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0008700	molecular_function	4-hydroxy-2-oxoglutarate aldolase activity
H	0008840	molecular_function	4-hydroxy-tetrahydrodipicolinate synthase activity
H	0009085	biological_process	lysine biosynthetic process
H	0009089	biological_process	lysine biosynthetic process via diaminopimelate
H	0009436	biological_process	glyoxylate catabolic process
H	0016829	molecular_function	lyase activity
H	0019877	biological_process	diaminopimelate biosynthetic process
H	0044281	biological_process	small molecule metabolic process
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0008700	molecular_function	4-hydroxy-2-oxoglutarate aldolase activity
I	0008840	molecular_function	4-hydroxy-tetrahydrodipicolinate synthase activity
I	0009085	biological_process	lysine biosynthetic process
I	0009089	biological_process	lysine biosynthetic process via diaminopimelate
I	0009436	biological_process	glyoxylate catabolic process
I	0016829	molecular_function	lyase activity
I	0019877	biological_process	diaminopimelate biosynthetic process
I	0044281	biological_process	small molecule metabolic process
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0008700	molecular_function	4-hydroxy-2-oxoglutarate aldolase activity
J	0008840	molecular_function	4-hydroxy-tetrahydrodipicolinate synthase activity
J	0009085	biological_process	lysine biosynthetic process
J	0009089	biological_process	lysine biosynthetic process via diaminopimelate
J	0009436	biological_process	glyoxylate catabolic process
J	0016829	molecular_function	lyase activity
J	0019877	biological_process	diaminopimelate biosynthetic process
J	0044281	biological_process	small molecule metabolic process
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0008700	molecular_function	4-hydroxy-2-oxoglutarate aldolase activity
K	0008840	molecular_function	4-hydroxy-tetrahydrodipicolinate synthase activity
K	0009085	biological_process	lysine biosynthetic process
K	0009089	biological_process	lysine biosynthetic process via diaminopimelate
K	0009436	biological_process	glyoxylate catabolic process
K	0016829	molecular_function	lyase activity
K	0019877	biological_process	diaminopimelate biosynthetic process
K	0044281	biological_process	small molecule metabolic process
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0008700	molecular_function	4-hydroxy-2-oxoglutarate aldolase activity
L	0008840	molecular_function	4-hydroxy-tetrahydrodipicolinate synthase activity
L	0009085	biological_process	lysine biosynthetic process
L	0009089	biological_process	lysine biosynthetic process via diaminopimelate
L	0009436	biological_process	glyoxylate catabolic process
L	0016829	molecular_function	lyase activity
L	0019877	biological_process	diaminopimelate biosynthetic process
L	0044281	biological_process	small molecule metabolic process

Functional Information from PROSITE/UniProt

site_id	PS00665
Number of Residues	18
Details	DHDPS_1 Dihydrodipicolinate synthase signature 1. AVVpvGTTGESatltheE

Chain	Residue	Details
A	ALA41-GLU58

site_id	PS00666
Number of Residues	32
Details	DHDPS_2 Dihydrodipicolinate synthase signature 2. YNVPgrTgceIstdtiiklfrdcenIyGVKEA

Chain	Residue	Details
A	TYR137-ALA168

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	ACT_SITE: Proton donor/acceptor => ECO:0000255\|HAMAP-Rule:MF_00418

Chain	Residue	Details
A	TYR137
B	TYR137
C	TYR137
D	TYR137
E	TYR137
F	TYR137
G	TYR137
H	TYR137
I	TYR137
J	TYR137
K	TYR137
L	TYR137

site_id	SWS_FT_FI2
Number of Residues	12
Details	ACT_SITE: Schiff-base intermediate with substrate => ECO:0000255\|HAMAP-Rule:MF_00418

Chain	Residue	Details
A	KPI166
B	KPI166
C	KPI166
D	KPI166
E	KPI166
F	KPI166
G	KPI166
H	KPI166
I	KPI166
J	KPI166
K	KPI166
L	KPI166

site_id	SWS_FT_FI3
Number of Residues	24
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_00418

Chain	Residue	Details
A	THR48
A	ILE207
B	THR48
B	ILE207
C	THR48
C	ILE207
D	THR48
D	ILE207
E	THR48
E	ILE207
F	THR48
F	ILE207
G	THR48
G	ILE207
H	THR48
H	ILE207
I	THR48
I	ILE207
J	THR48
J	ILE207
K	THR48
K	ILE207
L	THR48
L	ILE207

site_id	SWS_FT_FI4
Number of Residues	24
Details	SITE: Part of a proton relay during catalysis => ECO:0000255\|HAMAP-Rule:MF_00418

Chain	Residue	Details
A	THR47
A	TYR111
B	THR47
B	TYR111
C	THR47
C	TYR111
D	THR47
D	TYR111
E	THR47
E	TYR111
F	THR47
F	TYR111
G	THR47
G	TYR111
H	THR47
H	TYR111
I	THR47
I	TYR111
J	THR47
J	TYR111
K	THR47
K	TYR111
L	THR47
L	TYR111

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)