7CR7

human KCNQ2-CaM in complex with retigabine

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005216	molecular_function	monoatomic ion channel activity
A	0005249	molecular_function	voltage-gated potassium channel activity
A	0006811	biological_process	monoatomic ion transport
A	0006813	biological_process	potassium ion transport
A	0016020	cellular_component	membrane
A	0055085	biological_process	transmembrane transport
B	0005216	molecular_function	monoatomic ion channel activity
B	0005249	molecular_function	voltage-gated potassium channel activity
B	0006811	biological_process	monoatomic ion transport
B	0006813	biological_process	potassium ion transport
B	0016020	cellular_component	membrane
B	0055085	biological_process	transmembrane transport
C	0000086	biological_process	G2/M transition of mitotic cell cycle
C	0000922	cellular_component	spindle pole
C	0002027	biological_process	regulation of heart rate
C	0005509	molecular_function	calcium ion binding
C	0005513	biological_process	detection of calcium ion
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005813	cellular_component	centrosome
C	0005819	cellular_component	spindle
C	0005856	cellular_component	cytoskeleton
C	0005876	cellular_component	spindle microtubule
C	0005886	cellular_component	plasma membrane
C	0007186	biological_process	G protein-coupled receptor signaling pathway
C	0008076	cellular_component	voltage-gated potassium channel complex
C	0008179	molecular_function	adenylate cyclase binding
C	0010800	biological_process	positive regulation of peptidyl-threonine phosphorylation
C	0010801	biological_process	negative regulation of peptidyl-threonine phosphorylation
C	0010856	molecular_function	adenylate cyclase activator activity
C	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
C	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
C	0016020	cellular_component	membrane
C	0019901	molecular_function	protein kinase binding
C	0021762	biological_process	substantia nigra development
C	0030017	cellular_component	sarcomere
C	0031432	molecular_function	titin binding
C	0031954	biological_process	positive regulation of protein autophosphorylation
C	0031982	cellular_component	vesicle
C	0032465	biological_process	regulation of cytokinesis
C	0032516	biological_process	positive regulation of phosphoprotein phosphatase activity
C	0032991	cellular_component	protein-containing complex
C	0034704	cellular_component	calcium channel complex
C	0035307	biological_process	positive regulation of protein dephosphorylation
C	0043209	cellular_component	myelin sheath
C	0043539	molecular_function	protein serine/threonine kinase activator activity
C	0044325	molecular_function	transmembrane transporter binding
C	0046872	molecular_function	metal ion binding
C	0048306	molecular_function	calcium-dependent protein binding
C	0050848	biological_process	regulation of calcium-mediated signaling
C	0051343	biological_process	positive regulation of cyclic-nucleotide phosphodiesterase activity
C	0051592	biological_process	response to calcium ion
C	0055117	biological_process	regulation of cardiac muscle contraction
C	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
C	0060316	biological_process	positive regulation of ryanodine-sensitive calcium-release channel activity
C	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
C	0072542	molecular_function	protein phosphatase activator activity
C	0097225	cellular_component	sperm midpiece
C	0098901	biological_process	regulation of cardiac muscle cell action potential
C	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
C	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
C	1902494	cellular_component	catalytic complex
C	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
D	0005216	molecular_function	monoatomic ion channel activity
D	0005249	molecular_function	voltage-gated potassium channel activity
D	0006811	biological_process	monoatomic ion transport
D	0006813	biological_process	potassium ion transport
D	0016020	cellular_component	membrane
D	0055085	biological_process	transmembrane transport
E	0000086	biological_process	G2/M transition of mitotic cell cycle
E	0000922	cellular_component	spindle pole
E	0002027	biological_process	regulation of heart rate
E	0005509	molecular_function	calcium ion binding
E	0005513	biological_process	detection of calcium ion
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005813	cellular_component	centrosome
E	0005819	cellular_component	spindle
E	0005856	cellular_component	cytoskeleton
E	0005876	cellular_component	spindle microtubule
E	0005886	cellular_component	plasma membrane
E	0007186	biological_process	G protein-coupled receptor signaling pathway
E	0008076	cellular_component	voltage-gated potassium channel complex
E	0008179	molecular_function	adenylate cyclase binding
E	0010800	biological_process	positive regulation of peptidyl-threonine phosphorylation
E	0010801	biological_process	negative regulation of peptidyl-threonine phosphorylation
E	0010856	molecular_function	adenylate cyclase activator activity
E	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
E	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
E	0016020	cellular_component	membrane
E	0019901	molecular_function	protein kinase binding
E	0021762	biological_process	substantia nigra development
E	0030017	cellular_component	sarcomere
E	0031432	molecular_function	titin binding
E	0031954	biological_process	positive regulation of protein autophosphorylation
E	0031982	cellular_component	vesicle
E	0032465	biological_process	regulation of cytokinesis
E	0032516	biological_process	positive regulation of phosphoprotein phosphatase activity
E	0032991	cellular_component	protein-containing complex
E	0034704	cellular_component	calcium channel complex
E	0035307	biological_process	positive regulation of protein dephosphorylation
E	0043209	cellular_component	myelin sheath
E	0043539	molecular_function	protein serine/threonine kinase activator activity
E	0044325	molecular_function	transmembrane transporter binding
E	0046872	molecular_function	metal ion binding
E	0048306	molecular_function	calcium-dependent protein binding
E	0050848	biological_process	regulation of calcium-mediated signaling
E	0051343	biological_process	positive regulation of cyclic-nucleotide phosphodiesterase activity
E	0051592	biological_process	response to calcium ion
E	0055117	biological_process	regulation of cardiac muscle contraction
E	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
E	0060316	biological_process	positive regulation of ryanodine-sensitive calcium-release channel activity
E	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
E	0072542	molecular_function	protein phosphatase activator activity
E	0097225	cellular_component	sperm midpiece
E	0098901	biological_process	regulation of cardiac muscle cell action potential
E	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
E	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
E	1902494	cellular_component	catalytic complex
E	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
F	0000086	biological_process	G2/M transition of mitotic cell cycle
F	0000922	cellular_component	spindle pole
F	0002027	biological_process	regulation of heart rate
F	0005509	molecular_function	calcium ion binding
F	0005513	biological_process	detection of calcium ion
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005813	cellular_component	centrosome
F	0005819	cellular_component	spindle
F	0005856	cellular_component	cytoskeleton
F	0005876	cellular_component	spindle microtubule
F	0005886	cellular_component	plasma membrane
F	0007186	biological_process	G protein-coupled receptor signaling pathway
F	0008076	cellular_component	voltage-gated potassium channel complex
F	0008179	molecular_function	adenylate cyclase binding
F	0010800	biological_process	positive regulation of peptidyl-threonine phosphorylation
F	0010801	biological_process	negative regulation of peptidyl-threonine phosphorylation
F	0010856	molecular_function	adenylate cyclase activator activity
F	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
F	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
F	0016020	cellular_component	membrane
F	0019901	molecular_function	protein kinase binding
F	0021762	biological_process	substantia nigra development
F	0030017	cellular_component	sarcomere
F	0031432	molecular_function	titin binding
F	0031954	biological_process	positive regulation of protein autophosphorylation
F	0031982	cellular_component	vesicle
F	0032465	biological_process	regulation of cytokinesis
F	0032516	biological_process	positive regulation of phosphoprotein phosphatase activity
F	0032991	cellular_component	protein-containing complex
F	0034704	cellular_component	calcium channel complex
F	0035307	biological_process	positive regulation of protein dephosphorylation
F	0043209	cellular_component	myelin sheath
F	0043539	molecular_function	protein serine/threonine kinase activator activity
F	0044325	molecular_function	transmembrane transporter binding
F	0046872	molecular_function	metal ion binding
F	0048306	molecular_function	calcium-dependent protein binding
F	0050848	biological_process	regulation of calcium-mediated signaling
F	0051343	biological_process	positive regulation of cyclic-nucleotide phosphodiesterase activity
F	0051592	biological_process	response to calcium ion
F	0055117	biological_process	regulation of cardiac muscle contraction
F	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
F	0060316	biological_process	positive regulation of ryanodine-sensitive calcium-release channel activity
F	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
F	0072542	molecular_function	protein phosphatase activator activity
F	0097225	cellular_component	sperm midpiece
F	0098901	biological_process	regulation of cardiac muscle cell action potential
F	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
F	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
F	1902494	cellular_component	catalytic complex
F	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
G	0005216	molecular_function	monoatomic ion channel activity
G	0005249	molecular_function	voltage-gated potassium channel activity
G	0006811	biological_process	monoatomic ion transport
G	0006813	biological_process	potassium ion transport
G	0016020	cellular_component	membrane
G	0055085	biological_process	transmembrane transport
H	0000086	biological_process	G2/M transition of mitotic cell cycle
H	0000922	cellular_component	spindle pole
H	0002027	biological_process	regulation of heart rate
H	0005509	molecular_function	calcium ion binding
H	0005513	biological_process	detection of calcium ion
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005737	cellular_component	cytoplasm
H	0005813	cellular_component	centrosome
H	0005819	cellular_component	spindle
H	0005856	cellular_component	cytoskeleton
H	0005876	cellular_component	spindle microtubule
H	0005886	cellular_component	plasma membrane
H	0007186	biological_process	G protein-coupled receptor signaling pathway
H	0008076	cellular_component	voltage-gated potassium channel complex
H	0008179	molecular_function	adenylate cyclase binding
H	0010800	biological_process	positive regulation of peptidyl-threonine phosphorylation
H	0010801	biological_process	negative regulation of peptidyl-threonine phosphorylation
H	0010856	molecular_function	adenylate cyclase activator activity
H	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
H	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
H	0016020	cellular_component	membrane
H	0019901	molecular_function	protein kinase binding
H	0021762	biological_process	substantia nigra development
H	0030017	cellular_component	sarcomere
H	0031432	molecular_function	titin binding
H	0031954	biological_process	positive regulation of protein autophosphorylation
H	0031982	cellular_component	vesicle
H	0032465	biological_process	regulation of cytokinesis
H	0032516	biological_process	positive regulation of phosphoprotein phosphatase activity
H	0032991	cellular_component	protein-containing complex
H	0034704	cellular_component	calcium channel complex
H	0035307	biological_process	positive regulation of protein dephosphorylation
H	0043209	cellular_component	myelin sheath
H	0043539	molecular_function	protein serine/threonine kinase activator activity
H	0044325	molecular_function	transmembrane transporter binding
H	0046872	molecular_function	metal ion binding
H	0048306	molecular_function	calcium-dependent protein binding
H	0050848	biological_process	regulation of calcium-mediated signaling
H	0051343	biological_process	positive regulation of cyclic-nucleotide phosphodiesterase activity
H	0051592	biological_process	response to calcium ion
H	0055117	biological_process	regulation of cardiac muscle contraction
H	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
H	0060316	biological_process	positive regulation of ryanodine-sensitive calcium-release channel activity
H	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
H	0072542	molecular_function	protein phosphatase activator activity
H	0097225	cellular_component	sperm midpiece
H	0098901	biological_process	regulation of cardiac muscle cell action potential
H	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
H	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
H	1902494	cellular_component	catalytic complex
H	1905913	biological_process	negative regulation of calcium ion export across plasma membrane

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	binding site for residue FBX A 1001

Chain	Residue
A	TRP236
A	PHE240
A	PHE305
G	LEU299
G	ILE300
G	SER303
G	PHE304

---

site_id	AC2
Number of Residues	6
Details	binding site for residue FBX B 1001

Chain	Residue
A	SER303
B	TRP236
B	PHE240
B	PHE305
A	LEU299
A	ILE300

---

site_id	AC3
Number of Residues	6
Details	binding site for residue FBX D 1001

Chain	Residue
B	LEU299
B	ILE300
B	SER303
D	TRP236
D	PHE240
D	PHE305

---

site_id	AC4
Number of Residues	6
Details	binding site for residue FBX G 1001

Chain	Residue
D	LEU299
D	ILE300
D	SER303
G	TRP236
G	PHE240
G	PHE305

---

Functional Information from PROSITE/UniProt

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DKDGDGTITtkEL

Chain	Residue	Details
F	ASP21-LEU33
F	ASP57-PHE69
F	ASP94-LEU106
F	ASP130-PHE142

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	80
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0000269|PubMed:1474585

Chain	Residue	Details
F	ASP21
F	GLU68
F	ASP94
F	ASP96
F	ASN98
F	TYR100
F	GLU105
F	ASP130
F	ASP132
F	ASP134
F	GLN136
F	ASP23
F	GLU141
C	ASP21
C	ASP23
C	ASP25
C	THR27
C	GLU32
C	ASP57
C	ASP59
C	ASN61
C	THR63
F	ASP25
C	GLU68
C	ASP94
C	ASP96
C	ASN98
C	TYR100
C	GLU105
C	ASP130
C	ASP132
C	ASP134
C	GLN136
F	THR27
C	GLU141
E	ASP21
E	ASP23
E	ASP25
E	THR27
E	GLU32
E	ASP57
E	ASP59
E	ASN61
E	THR63
F	GLU32
E	GLU68
E	ASP94
E	ASP96
E	ASN98
E	TYR100
E	GLU105
E	ASP130
E	ASP132
E	ASP134
E	GLN136
F	ASP57
E	GLU141
H	ASP21
H	ASP23
H	ASP25
H	THR27
H	GLU32
H	ASP57
H	ASP59
H	ASN61
H	THR63
F	ASP59
H	GLU68
H	ASP94
H	ASP96
H	ASN98
H	TYR100
H	GLU105
H	ASP130
H	ASP132
H	ASP134
H	GLN136
F	ASN61
H	GLU141
F	THR63

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	MOD_RES: N-acetylalanine => ECO:0000269|PubMed:7093203, ECO:0000269|Ref.7, ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378, ECO:0007744|PubMed:25944712

Chain	Residue	Details
F	ALA2
D	GLN188-SER195
D	ALA253-TYR264
D	GLN286-ARG291
G	SER113-SER122
G	GLN188-SER195
G	ALA253-TYR264
G	GLN286-ARG291
C	ALA2
E	ALA2
H	ALA2
B	SER113-SER122
B	GLN188-SER195
B	ALA253-TYR264
B	GLN286-ARG291
D	SER113-SER122

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0007744|PubMed:19608861

Chain	Residue	Details
F	LYS22
C	LYS22
E	LYS22
H	LYS22

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: Phosphothreonine; by CaMK4 => ECO:0000250|UniProtKB:P0DP31

Chain	Residue	Details
F	THR45
C	THR45
E	THR45
H	THR45
D	ARG144-LYS166
D	LYS219-GLU231
G	ARG144-LYS166
G	LYS219-GLU231

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
F	SER82
C	SER82
E	SER82
H	SER82

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
F	LYS95
C	LYS95
E	LYS95
H	LYS95

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332

Chain	Residue	Details
F	TYR100
C	TYR100
E	TYR100
H	TYR100

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569

Chain	Residue	Details
F	SER102
C	SER102
E	SER102
H	SER102

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:24275569

Chain	Residue	Details
F	THR111
C	THR111
E	THR111
H	THR111

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	MOD_RES: N6-methyllysine; alternate => ECO:0007744|PubMed:24129315

Chain	Residue	Details
F	LYS116
C	LYS116
E	LYS116
H	LYS116

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:19690332

Chain	Residue	Details
F	TYR139
B	SER478
D	SER466
D	SER468
D	SER472
D	SER476
D	SER478
G	SER466
G	SER468
G	SER472
G	SER476
C	TYR139
G	SER478
E	TYR139
H	TYR139
A	SER478
B	SER466
B	SER468
B	SER472
B	SER476

---

site_id	SWS_FT_FI12
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250|UniProtKB:P62157

Chain	Residue	Details
F	LYS22
A	SER672
C	LYS22
B	SER672
E	LYS22
D	SER672
H	LYS22
G	SER672

---