Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7B5G

Crystal structure of E.coli LexA in complex with nanobody NbSOS3(Nb14527)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000976	molecular_function	transcription cis-regulatory region binding
A	0001217	molecular_function	DNA-binding transcription repressor activity
A	0003677	molecular_function	DNA binding
A	0004252	molecular_function	serine-type endopeptidase activity
A	0005515	molecular_function	protein binding
A	0005829	cellular_component	cytosol
A	0006260	biological_process	DNA replication
A	0006281	biological_process	DNA repair
A	0006351	biological_process	DNA-templated transcription
A	0006355	biological_process	regulation of DNA-templated transcription
A	0006508	biological_process	proteolysis
A	0006974	biological_process	DNA damage response
A	0009432	biological_process	SOS response
A	0016787	molecular_function	hydrolase activity
A	0032993	cellular_component	protein-DNA complex
A	0042802	molecular_function	identical protein binding
A	0045892	biological_process	negative regulation of DNA-templated transcription
C	0000976	molecular_function	transcription cis-regulatory region binding
C	0001217	molecular_function	DNA-binding transcription repressor activity
C	0003677	molecular_function	DNA binding
C	0004252	molecular_function	serine-type endopeptidase activity
C	0005515	molecular_function	protein binding
C	0005829	cellular_component	cytosol
C	0006260	biological_process	DNA replication
C	0006281	biological_process	DNA repair
C	0006351	biological_process	DNA-templated transcription
C	0006355	biological_process	regulation of DNA-templated transcription
C	0006508	biological_process	proteolysis
C	0006974	biological_process	DNA damage response
C	0009432	biological_process	SOS response
C	0016787	molecular_function	hydrolase activity
C	0032993	cellular_component	protein-DNA complex
C	0042802	molecular_function	identical protein binding
C	0045892	biological_process	negative regulation of DNA-templated transcription
E	0000976	molecular_function	transcription cis-regulatory region binding
E	0001217	molecular_function	DNA-binding transcription repressor activity
E	0003677	molecular_function	DNA binding
E	0004252	molecular_function	serine-type endopeptidase activity
E	0005515	molecular_function	protein binding
E	0005829	cellular_component	cytosol
E	0006260	biological_process	DNA replication
E	0006281	biological_process	DNA repair
E	0006351	biological_process	DNA-templated transcription
E	0006355	biological_process	regulation of DNA-templated transcription
E	0006508	biological_process	proteolysis
E	0006974	biological_process	DNA damage response
E	0009432	biological_process	SOS response
E	0016787	molecular_function	hydrolase activity
E	0032993	cellular_component	protein-DNA complex
E	0042802	molecular_function	identical protein binding
E	0045892	biological_process	negative regulation of DNA-templated transcription
G	0000976	molecular_function	transcription cis-regulatory region binding
G	0001217	molecular_function	DNA-binding transcription repressor activity
G	0003677	molecular_function	DNA binding
G	0004252	molecular_function	serine-type endopeptidase activity
G	0005515	molecular_function	protein binding
G	0005829	cellular_component	cytosol
G	0006260	biological_process	DNA replication
G	0006281	biological_process	DNA repair
G	0006351	biological_process	DNA-templated transcription
G	0006355	biological_process	regulation of DNA-templated transcription
G	0006508	biological_process	proteolysis
G	0006974	biological_process	DNA damage response
G	0009432	biological_process	SOS response
G	0016787	molecular_function	hydrolase activity
G	0032993	cellular_component	protein-DNA complex
G	0042802	molecular_function	identical protein binding
G	0045892	biological_process	negative regulation of DNA-templated transcription

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	80
Details	DNA_BIND: H-T-H motif => ECO:0000255\|HAMAP-Rule:MF_00015

Chain	Residue	Details
A	ARG28-LYS48
C	ARG28-LYS48
E	ARG28-LYS48
G	ARG28-LYS48

site_id	SWS_FT_FI2
Number of Residues	8
Details	ACT_SITE: For autocatalytic cleavage activity => ECO:0000255\|HAMAP-Rule:MF_00015

Chain	Residue	Details
A	SER119
A	LYS156
C	SER119
C	LYS156
E	SER119
E	LYS156
G	SER119
G	LYS156

site_id	SWS_FT_FI3
Number of Residues	4
Details	SITE: Cleavage; by autolysis

Chain	Residue	Details
A	ALA84
C	ALA84
E	ALA84
G	ALA84

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	4
Details	M-CSA 724

Chain	Residue	Details
A	MET118	electrostatic stabiliser
A	SER119	electrostatic stabiliser
A	GLU152	electrostatic stabiliser
A	LYS156	proton acceptor, proton donor

site_id	MCSA2
Number of Residues	4
Details	M-CSA 724

Chain	Residue	Details
C	MET118	electrostatic stabiliser
C	SER119	electrostatic stabiliser
C	GLU152	electrostatic stabiliser
C	LYS156	proton acceptor, proton donor

site_id	MCSA3
Number of Residues	4
Details	M-CSA 724

Chain	Residue	Details
E	MET118	electrostatic stabiliser
E	SER119	electrostatic stabiliser
E	GLU152	electrostatic stabiliser
E	LYS156	proton acceptor, proton donor

site_id	MCSA4
Number of Residues	4
Details	M-CSA 724

Chain	Residue	Details
G	MET118	electrostatic stabiliser
G	SER119	electrostatic stabiliser
G	GLU152	electrostatic stabiliser
G	LYS156	proton acceptor, proton donor

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)