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6SB3

CryoEM structure of murine perforin-2 ectodomain in a pre-pore form

Functional Information from GO Data
ChainGOidnamespacecontents
A0002250biological_processadaptive immune response
A0002468biological_processdendritic cell antigen processing and presentation
A0002478biological_processantigen processing and presentation of exogenous peptide antigen
A0016020cellular_componentmembrane
A0022829molecular_functionwide pore channel activity
A0030670cellular_componentphagocytic vesicle membrane
A0031410cellular_componentcytoplasmic vesicle
A0036019cellular_componentendolysosome
A0042590biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I
A0042742biological_processdefense response to bacterium
A0045087biological_processinnate immune response
A0045335cellular_componentphagocytic vesicle
A0050829biological_processdefense response to Gram-negative bacterium
A0050830biological_processdefense response to Gram-positive bacterium
A0055085biological_processtransmembrane transport
A0061474cellular_componentphagolysosome membrane
A0140367biological_processantibacterial innate immune response
A0140911molecular_functionpore-forming activity
B0002250biological_processadaptive immune response
B0002468biological_processdendritic cell antigen processing and presentation
B0002478biological_processantigen processing and presentation of exogenous peptide antigen
B0016020cellular_componentmembrane
B0022829molecular_functionwide pore channel activity
B0030670cellular_componentphagocytic vesicle membrane
B0031410cellular_componentcytoplasmic vesicle
B0036019cellular_componentendolysosome
B0042590biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I
B0042742biological_processdefense response to bacterium
B0045087biological_processinnate immune response
B0045335cellular_componentphagocytic vesicle
B0050829biological_processdefense response to Gram-negative bacterium
B0050830biological_processdefense response to Gram-positive bacterium
B0055085biological_processtransmembrane transport
B0061474cellular_componentphagolysosome membrane
B0140367biological_processantibacterial innate immune response
B0140911molecular_functionpore-forming activity
C0002250biological_processadaptive immune response
C0002468biological_processdendritic cell antigen processing and presentation
C0002478biological_processantigen processing and presentation of exogenous peptide antigen
C0016020cellular_componentmembrane
C0022829molecular_functionwide pore channel activity
C0030670cellular_componentphagocytic vesicle membrane
C0031410cellular_componentcytoplasmic vesicle
C0036019cellular_componentendolysosome
C0042590biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I
C0042742biological_processdefense response to bacterium
C0045087biological_processinnate immune response
C0045335cellular_componentphagocytic vesicle
C0050829biological_processdefense response to Gram-negative bacterium
C0050830biological_processdefense response to Gram-positive bacterium
C0055085biological_processtransmembrane transport
C0061474cellular_componentphagolysosome membrane
C0140367biological_processantibacterial innate immune response
C0140911molecular_functionpore-forming activity
D0002250biological_processadaptive immune response
D0002468biological_processdendritic cell antigen processing and presentation
D0002478biological_processantigen processing and presentation of exogenous peptide antigen
D0016020cellular_componentmembrane
D0022829molecular_functionwide pore channel activity
D0030670cellular_componentphagocytic vesicle membrane
D0031410cellular_componentcytoplasmic vesicle
D0036019cellular_componentendolysosome
D0042590biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I
D0042742biological_processdefense response to bacterium
D0045087biological_processinnate immune response
D0045335cellular_componentphagocytic vesicle
D0050829biological_processdefense response to Gram-negative bacterium
D0050830biological_processdefense response to Gram-positive bacterium
D0055085biological_processtransmembrane transport
D0061474cellular_componentphagolysosome membrane
D0140367biological_processantibacterial innate immune response
D0140911molecular_functionpore-forming activity
E0002250biological_processadaptive immune response
E0002468biological_processdendritic cell antigen processing and presentation
E0002478biological_processantigen processing and presentation of exogenous peptide antigen
E0016020cellular_componentmembrane
E0022829molecular_functionwide pore channel activity
E0030670cellular_componentphagocytic vesicle membrane
E0031410cellular_componentcytoplasmic vesicle
E0036019cellular_componentendolysosome
E0042590biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I
E0042742biological_processdefense response to bacterium
E0045087biological_processinnate immune response
E0045335cellular_componentphagocytic vesicle
E0050829biological_processdefense response to Gram-negative bacterium
E0050830biological_processdefense response to Gram-positive bacterium
E0055085biological_processtransmembrane transport
E0061474cellular_componentphagolysosome membrane
E0140367biological_processantibacterial innate immune response
E0140911molecular_functionpore-forming activity
F0002250biological_processadaptive immune response
F0002468biological_processdendritic cell antigen processing and presentation
F0002478biological_processantigen processing and presentation of exogenous peptide antigen
F0016020cellular_componentmembrane
F0022829molecular_functionwide pore channel activity
F0030670cellular_componentphagocytic vesicle membrane
F0031410cellular_componentcytoplasmic vesicle
F0036019cellular_componentendolysosome
F0042590biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I
F0042742biological_processdefense response to bacterium
F0045087biological_processinnate immune response
F0045335cellular_componentphagocytic vesicle
F0050829biological_processdefense response to Gram-negative bacterium
F0050830biological_processdefense response to Gram-positive bacterium
F0055085biological_processtransmembrane transport
F0061474cellular_componentphagolysosome membrane
F0140367biological_processantibacterial innate immune response
F0140911molecular_functionpore-forming activity
G0002250biological_processadaptive immune response
G0002468biological_processdendritic cell antigen processing and presentation
G0002478biological_processantigen processing and presentation of exogenous peptide antigen
G0016020cellular_componentmembrane
G0022829molecular_functionwide pore channel activity
G0030670cellular_componentphagocytic vesicle membrane
G0031410cellular_componentcytoplasmic vesicle
G0036019cellular_componentendolysosome
G0042590biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I
G0042742biological_processdefense response to bacterium
G0045087biological_processinnate immune response
G0045335cellular_componentphagocytic vesicle
G0050829biological_processdefense response to Gram-negative bacterium
G0050830biological_processdefense response to Gram-positive bacterium
G0055085biological_processtransmembrane transport
G0061474cellular_componentphagolysosome membrane
G0140367biological_processantibacterial innate immune response
G0140911molecular_functionpore-forming activity
H0002250biological_processadaptive immune response
H0002468biological_processdendritic cell antigen processing and presentation
H0002478biological_processantigen processing and presentation of exogenous peptide antigen
H0016020cellular_componentmembrane
H0022829molecular_functionwide pore channel activity
H0030670cellular_componentphagocytic vesicle membrane
H0031410cellular_componentcytoplasmic vesicle
H0036019cellular_componentendolysosome
H0042590biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I
H0042742biological_processdefense response to bacterium
H0045087biological_processinnate immune response
H0045335cellular_componentphagocytic vesicle
H0050829biological_processdefense response to Gram-negative bacterium
H0050830biological_processdefense response to Gram-positive bacterium
H0055085biological_processtransmembrane transport
H0061474cellular_componentphagolysosome membrane
H0140367biological_processantibacterial innate immune response
H0140911molecular_functionpore-forming activity
I0002250biological_processadaptive immune response
I0002468biological_processdendritic cell antigen processing and presentation
I0002478biological_processantigen processing and presentation of exogenous peptide antigen
I0016020cellular_componentmembrane
I0022829molecular_functionwide pore channel activity
I0030670cellular_componentphagocytic vesicle membrane
I0031410cellular_componentcytoplasmic vesicle
I0036019cellular_componentendolysosome
I0042590biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I
I0042742biological_processdefense response to bacterium
I0045087biological_processinnate immune response
I0045335cellular_componentphagocytic vesicle
I0050829biological_processdefense response to Gram-negative bacterium
I0050830biological_processdefense response to Gram-positive bacterium
I0055085biological_processtransmembrane transport
I0061474cellular_componentphagolysosome membrane
I0140367biological_processantibacterial innate immune response
I0140911molecular_functionpore-forming activity
J0002250biological_processadaptive immune response
J0002468biological_processdendritic cell antigen processing and presentation
J0002478biological_processantigen processing and presentation of exogenous peptide antigen
J0016020cellular_componentmembrane
J0022829molecular_functionwide pore channel activity
J0030670cellular_componentphagocytic vesicle membrane
J0031410cellular_componentcytoplasmic vesicle
J0036019cellular_componentendolysosome
J0042590biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I
J0042742biological_processdefense response to bacterium
J0045087biological_processinnate immune response
J0045335cellular_componentphagocytic vesicle
J0050829biological_processdefense response to Gram-negative bacterium
J0050830biological_processdefense response to Gram-positive bacterium
J0055085biological_processtransmembrane transport
J0061474cellular_componentphagolysosome membrane
J0140367biological_processantibacterial innate immune response
J0140911molecular_functionpore-forming activity
K0002250biological_processadaptive immune response
K0002468biological_processdendritic cell antigen processing and presentation
K0002478biological_processantigen processing and presentation of exogenous peptide antigen
K0016020cellular_componentmembrane
K0022829molecular_functionwide pore channel activity
K0030670cellular_componentphagocytic vesicle membrane
K0031410cellular_componentcytoplasmic vesicle
K0036019cellular_componentendolysosome
K0042590biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I
K0042742biological_processdefense response to bacterium
K0045087biological_processinnate immune response
K0045335cellular_componentphagocytic vesicle
K0050829biological_processdefense response to Gram-negative bacterium
K0050830biological_processdefense response to Gram-positive bacterium
K0055085biological_processtransmembrane transport
K0061474cellular_componentphagolysosome membrane
K0140367biological_processantibacterial innate immune response
K0140911molecular_functionpore-forming activity
L0002250biological_processadaptive immune response
L0002468biological_processdendritic cell antigen processing and presentation
L0002478biological_processantigen processing and presentation of exogenous peptide antigen
L0016020cellular_componentmembrane
L0022829molecular_functionwide pore channel activity
L0030670cellular_componentphagocytic vesicle membrane
L0031410cellular_componentcytoplasmic vesicle
L0036019cellular_componentendolysosome
L0042590biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I
L0042742biological_processdefense response to bacterium
L0045087biological_processinnate immune response
L0045335cellular_componentphagocytic vesicle
L0050829biological_processdefense response to Gram-negative bacterium
L0050830biological_processdefense response to Gram-positive bacterium
L0055085biological_processtransmembrane transport
L0061474cellular_componentphagolysosome membrane
L0140367biological_processantibacterial innate immune response
L0140911molecular_functionpore-forming activity
M0002250biological_processadaptive immune response
M0002468biological_processdendritic cell antigen processing and presentation
M0002478biological_processantigen processing and presentation of exogenous peptide antigen
M0016020cellular_componentmembrane
M0022829molecular_functionwide pore channel activity
M0030670cellular_componentphagocytic vesicle membrane
M0031410cellular_componentcytoplasmic vesicle
M0036019cellular_componentendolysosome
M0042590biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I
M0042742biological_processdefense response to bacterium
M0045087biological_processinnate immune response
M0045335cellular_componentphagocytic vesicle
M0050829biological_processdefense response to Gram-negative bacterium
M0050830biological_processdefense response to Gram-positive bacterium
M0055085biological_processtransmembrane transport
M0061474cellular_componentphagolysosome membrane
M0140367biological_processantibacterial innate immune response
M0140911molecular_functionpore-forming activity
N0002250biological_processadaptive immune response
N0002468biological_processdendritic cell antigen processing and presentation
N0002478biological_processantigen processing and presentation of exogenous peptide antigen
N0016020cellular_componentmembrane
N0022829molecular_functionwide pore channel activity
N0030670cellular_componentphagocytic vesicle membrane
N0031410cellular_componentcytoplasmic vesicle
N0036019cellular_componentendolysosome
N0042590biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I
N0042742biological_processdefense response to bacterium
N0045087biological_processinnate immune response
N0045335cellular_componentphagocytic vesicle
N0050829biological_processdefense response to Gram-negative bacterium
N0050830biological_processdefense response to Gram-positive bacterium
N0055085biological_processtransmembrane transport
N0061474cellular_componentphagolysosome membrane
N0140367biological_processantibacterial innate immune response
N0140911molecular_functionpore-forming activity
O0002250biological_processadaptive immune response
O0002468biological_processdendritic cell antigen processing and presentation
O0002478biological_processantigen processing and presentation of exogenous peptide antigen
O0016020cellular_componentmembrane
O0022829molecular_functionwide pore channel activity
O0030670cellular_componentphagocytic vesicle membrane
O0031410cellular_componentcytoplasmic vesicle
O0036019cellular_componentendolysosome
O0042590biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I
O0042742biological_processdefense response to bacterium
O0045087biological_processinnate immune response
O0045335cellular_componentphagocytic vesicle
O0050829biological_processdefense response to Gram-negative bacterium
O0050830biological_processdefense response to Gram-positive bacterium
O0055085biological_processtransmembrane transport
O0061474cellular_componentphagolysosome membrane
O0140367biological_processantibacterial innate immune response
O0140911molecular_functionpore-forming activity
P0002250biological_processadaptive immune response
P0002468biological_processdendritic cell antigen processing and presentation
P0002478biological_processantigen processing and presentation of exogenous peptide antigen
P0016020cellular_componentmembrane
P0022829molecular_functionwide pore channel activity
P0030670cellular_componentphagocytic vesicle membrane
P0031410cellular_componentcytoplasmic vesicle
P0036019cellular_componentendolysosome
P0042590biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I
P0042742biological_processdefense response to bacterium
P0045087biological_processinnate immune response
P0045335cellular_componentphagocytic vesicle
P0050829biological_processdefense response to Gram-negative bacterium
P0050830biological_processdefense response to Gram-positive bacterium
P0055085biological_processtransmembrane transport
P0061474cellular_componentphagolysosome membrane
P0140367biological_processantibacterial innate immune response
P0140911molecular_functionpore-forming activity
Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues192
DetailsTRANSMEM: Beta stranded; Name=TMH1 => ECO:0000269|PubMed:36245269, ECO:0007744|PDB:8A1D, ECO:0007744|PDB:8A1S
ChainResidueDetails
PGLY127-GLU132
ALEU113-ALA120
AGLY127-GLU132
BLEU113-ALA120
BGLY127-GLU132
CLEU113-ALA120
CGLY127-GLU132
DLEU113-ALA120
DGLY127-GLU132
ELEU113-ALA120
EGLY127-GLU132
FLEU113-ALA120
FGLY127-GLU132
GLEU113-ALA120
GGLY127-GLU132
HLEU113-ALA120
HGLY127-GLU132
ILEU113-ALA120
IGLY127-GLU132
JLEU113-ALA120
JGLY127-GLU132
KLEU113-ALA120
KGLY127-GLU132
LLEU113-ALA120
LGLY127-GLU132
MLEU113-ALA120
MGLY127-GLU132
NLEU113-ALA120
NGLY127-GLU132
OLEU113-ALA120
OGLY127-GLU132
PLEU113-ALA120

site_idSWS_FT_FI2
Number of Residues272
DetailsTRANSMEM: Beta stranded; Name=TMH2 => ECO:0000269|PubMed:36245269, ECO:0007744|PDB:8A1D, ECO:0007744|PDB:8A1S
ChainResidueDetails
ATHR235-PHE244
AVAL248-TYR256
BTHR235-PHE244
BVAL248-TYR256
CTHR235-PHE244
CVAL248-TYR256
DTHR235-PHE244
DVAL248-TYR256
ETHR235-PHE244
EVAL248-TYR256
FTHR235-PHE244
FVAL248-TYR256
GTHR235-PHE244
GVAL248-TYR256
HTHR235-PHE244
HVAL248-TYR256
ITHR235-PHE244
IVAL248-TYR256
JTHR235-PHE244
JVAL248-TYR256
KTHR235-PHE244
KVAL248-TYR256
LTHR235-PHE244
LVAL248-TYR256
MTHR235-PHE244
MVAL248-TYR256
NTHR235-PHE244
NVAL248-TYR256
OTHR235-PHE244
OVAL248-TYR256
PTHR235-PHE244
PVAL248-TYR256

site_idSWS_FT_FI3
Number of Residues48
DetailsSITE: Cleavage; by LGMN => ECO:0000269|PubMed:37347855
ChainResidueDetails
AASN352
AASN357
AASN359
BASN352
BASN357
BASN359
CASN352
CASN357
CASN359
DASN352
DASN357
DASN359
EASN352
EASN357
EASN359
FASN352
FASN357
FASN359
GASN352
GASN357
GASN359
HASN352
HASN357
HASN359
IASN352
IASN357
IASN359
JASN352
JASN357
JASN359
KASN352
KASN357
KASN359
LASN352
LASN357
LASN359
MASN352
MASN357
MASN359
NASN352
NASN357
NASN359
OASN352
OASN357
OASN359
PASN352
PASN357
PASN359

site_idSWS_FT_FI4
Number of Residues16
DetailsSITE: Cleavage; by trypsin => ECO:0000305|PubMed:26402460, ECO:0000305|PubMed:37347855
ChainResidueDetails
ALYS628
BLYS628
CLYS628
DLYS628
ELYS628
FLYS628
GLYS628
HLYS628
ILYS628
JLYS628
KLYS628
LLYS628
MLYS628
NLYS628
OLYS628
PLYS628

site_idSWS_FT_FI5
Number of Residues16
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:32064340, ECO:0007744|PDB:6SB1, ECO:0007744|PDB:6SB3
ChainResidueDetails
AASN185
BASN185
CASN185
DASN185
EASN185
FASN185
GASN185
HASN185
IASN185
JASN185
KASN185
LASN185
MASN185
NASN185
OASN185
PASN185

site_idSWS_FT_FI6
Number of Residues16
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:32064340, ECO:0000269|PubMed:36245269, ECO:0007744|PDB:6SB1, ECO:0007744|PDB:6SB3, ECO:0007744|PDB:8A1D, ECO:0007744|PDB:8A1S
ChainResidueDetails
AASN269
BASN269
CASN269
DASN269
EASN269
FASN269
GASN269
HASN269
IASN269
JASN269
KASN269
LASN269
MASN269
NASN269
OASN269
PASN269

site_idSWS_FT_FI7
Number of Residues16
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255
ChainResidueDetails
AASN375
BASN375
CASN375
DASN375
EASN375
FASN375
GASN375
HASN375
IASN375
JASN375
KASN375
LASN375
MASN375
NASN375
OASN375
PASN375

219869

PDB entries from 2024-05-15

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