Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5Y1S

Crystal structure of Plasmodium falciparum aminopeptidase N in complex with (S)-2-(3-(3,4-dimethylbenzyl)ureido)-N-hydroxy-4-methylpentanamide

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0006508	biological_process	proteolysis
A	0008237	molecular_function	metallopeptidase activity
A	0008270	molecular_function	zinc ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue ZN A 1101

Chain	Residue
A	HIS496
A	HIS500
A	GLU519
A	E8F1105
A	E8R1106

site_id	AC2
Number of Residues	6
Details	binding site for residue MG A 1102

Chain	Residue
A	HOH1693
A	HOH1730
A	GLY250
A	HOH1302
A	HOH1403
A	HOH1677

site_id	AC3
Number of Residues	6
Details	binding site for residue MG A 1103

Chain	Residue
A	GLU526
A	HOH1458
A	HOH1459
A	HOH1656
A	HOH1673
A	HOH1744

site_id	AC4
Number of Residues	6
Details	binding site for residue MG A 1104

Chain	Residue
A	HOH1354
A	HOH1567
A	HOH1575
A	HOH1727
A	HOH1762
A	HOH1771

site_id	AC5
Number of Residues	20
Details	binding site for residue E8R A 1106

Chain	Residue
A	GLU319
A	VAL459
A	GLY460
A	ALA461
A	MET462
A	GLU463
A	ARG489
A	HIS496
A	GLU497
A	HIS500
A	GLU519
A	TYR580
A	ARG997
A	GLN1038
A	ZN1101
A	E8F1105
A	GOL1109
A	GOL1110
A	HOH1214
A	HOH1483

site_id	AC6
Number of Residues	9
Details	binding site for residue GOL A 1107

Chain	Residue
A	MET533
A	THR534
A	LYS535
A	HIS886
A	VAL887
A	ASP888
A	PHE889
A	ASP890
A	HOH1212

site_id	AC7
Number of Residues	6
Details	binding site for residue GOL A 1108

Chain	Residue
A	TYR853
A	SER903
A	LEU904
A	LYS907
A	HOH1209
A	HOH1679

site_id	AC8
Number of Residues	8
Details	binding site for residue GOL A 1109

Chain	Residue
A	TYR575
A	THR576
A	THR577
A	TYR580
A	E8F1105
A	E8R1106
A	GOL1110
A	HOH1231

site_id	AC9
Number of Residues	20
Details	binding site for residues E8F A 1105 and GOL A 1110

Chain	Residue
A	GLU319
A	VAL459
A	GLY460
A	ALA461
A	GLU463
A	ARG489
A	VAL493
A	HIS496
A	GLU497
A	HIS500
A	GLU519
A	GLU572
A	TYR575
A	TYR580
A	MET1034
A	ZN1101
A	E8R1106
A	GOL1109
A	HOH1214
A	HOH1656

site_id	AD1
Number of Residues	20
Details	binding site for residues E8F A 1105 and GOL A 1110

Chain	Residue
A	TYR580
A	MET1034
A	ZN1101
A	E8R1106
A	GOL1109
A	HOH1214
A	HOH1656
A	GLU319
A	VAL459
A	GLY460
A	ALA461
A	GLU463
A	ARG489
A	VAL493
A	HIS496
A	GLU497
A	HIS500
A	GLU519
A	GLU572
A	TYR575

site_id	AD2
Number of Residues	20
Details	binding site for residues E8F A 1105 and GOL A 1110

Chain	Residue
A	GLU319
A	VAL459
A	GLY460
A	ALA461
A	GLU463
A	ARG489
A	VAL493
A	HIS496
A	GLU497
A	HIS500
A	GLU519
A	GLU572
A	TYR575
A	TYR580
A	MET1034
A	ZN1101
A	E8R1106
A	GOL1109
A	HOH1214
A	HOH1656

site_id	AD3
Number of Residues	20
Details	binding site for residues E8F A 1105 and GOL A 1110

Chain	Residue
A	GLU319
A	VAL459
A	GLY460
A	ALA461
A	GLU463
A	ARG489
A	VAL493
A	HIS496
A	GLU497
A	HIS500
A	GLU519
A	GLU572
A	TYR575
A	TYR580
A	MET1034
A	ZN1101
A	E8R1106
A	GOL1109
A	HOH1214
A	HOH1656

site_id	AD4
Number of Residues	20
Details	binding site for residues E8F A 1105 and GOL A 1110

Chain	Residue
A	GLU319
A	VAL459
A	GLY460
A	ALA461
A	GLU463
A	ARG489
A	VAL493
A	HIS496
A	GLU497
A	HIS500
A	GLU519
A	GLU572
A	TYR575
A	TYR580
A	MET1034
A	ZN1101
A	E8R1106
A	GOL1109
A	HOH1214
A	HOH1656

site_id	AD5
Number of Residues	20
Details	binding site for residues E8F A 1105 and GOL A 1110

Chain	Residue
A	GLU319
A	VAL459
A	GLY460
A	ALA461
A	GLU463
A	ARG489
A	VAL493
A	HIS496
A	GLU497
A	HIS500
A	GLU519
A	GLU572
A	TYR575
A	TYR580
A	MET1034
A	ZN1101
A	E8R1106
A	GOL1109
A	HOH1214
A	HOH1656

site_id	AD6
Number of Residues	20
Details	binding site for residues E8F A 1105 and GOL A 1110

Chain	Residue
A	GLU319
A	VAL459
A	GLY460
A	ALA461
A	GLU463
A	ARG489
A	VAL493
A	HIS496
A	GLU497
A	HIS500
A	GLU519
A	GLU572
A	TYR575
A	TYR580
A	MET1034
A	ZN1101
A	E8R1106
A	GOL1109
A	HOH1214
A	HOH1656

Functional Information from PROSITE/UniProt

site_id	PS00142
Number of Residues	10
Details	ZINC_PROTEASE Neutral zinc metallopeptidases, zinc-binding region signature. VVGHEYFHNY

Chain	Residue	Details
A	VAL493-TYR502

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton acceptor => ECO:0000250\|UniProtKB:P15144

Chain	Residue	Details
A	GLU497

site_id	SWS_FT_FI2
Number of Residues	3
Details	BINDING: BINDING => ECO:0000305\|PubMed:19196988, ECO:0000305\|PubMed:21366301, ECO:0007744\|PDB:3EBH, ECO:0007744\|PDB:3EBI, ECO:0007744\|PDB:3Q43

Chain	Residue	Details
A	GLU319
A	GLY460
A	GLU463

site_id	SWS_FT_FI3
Number of Residues	1
Details	BINDING: BINDING => ECO:0000305\|PubMed:19196988, ECO:0000305\|PubMed:21366301, ECO:0007744\|PDB:3EBH, ECO:0007744\|PDB:3Q43

Chain	Residue	Details
A	ALA461

site_id	SWS_FT_FI4
Number of Residues	3
Details	BINDING: BINDING => ECO:0000269\|PubMed:19196988, ECO:0000269\|PubMed:21366301, ECO:0000269\|PubMed:21844374, ECO:0000269\|PubMed:23713488, ECO:0000269\|PubMed:23897806, ECO:0000269\|PubMed:25299353, ECO:0000269\|PubMed:25645579, ECO:0000269\|PubMed:26406322, ECO:0000269\|PubMed:26807544, ECO:0000269\|PubMed:30537832, ECO:0000269\|PubMed:32182520, ECO:0000269\|Ref.21, ECO:0000269\|DOI:10.1016/j.cclet.2021.09.102, ECO:0000312\|PDB:3T8V, ECO:0000312\|PDB:5Y1Q, ECO:0007744\|PDB:3EBG, ECO:0007744\|PDB:3EBH, ECO:0007744\|PDB:3EBI, ECO:0007744\|PDB:3Q43, ECO:0007744\|PDB:3Q44, ECO:0007744\|PDB:4J3B, ECO:0007744\|PDB:4K5L, ECO:0007744\|PDB:4K5M, ECO:0007744\|PDB:4K5N, ECO:0007744\|PDB:4K5O, ECO:0007744\|PDB:4K5P, ECO:0007744\|PDB:4R5T, ECO:0007744\|PDB:4R5V, ECO:0007744\|PDB:4R5X, ECO:0007744\|PDB:4X2U, ECO:0007744\|PDB:4ZQT, ECO:0007744\|PDB:4ZW3, ECO:0007744\|PDB:4ZW5, ECO:0007744\|PDB:4ZW6, ECO:0007744\|PDB:4ZW7, ECO:0007744\|PDB:4ZW8, ECO:0007744\|PDB:4ZX3, ECO:0007744\|PDB:4ZX4, ECO:0007744\|PDB:4ZX5, ECO:0007744\|PDB:4ZX6, ECO:0007744\|PDB:5XM7, ECO:0007744\|PDB:5Y19, ECO:0007744\|PDB:5Y1H, ECO:0007744\|PDB:5Y1K, ECO:0007744\|PDB:5Y1R, ECO:0007744\|PDB:5Y1S, ECO:0007744\|PDB:5Y1T, ECO:0007744\|PDB:5Y1V, ECO:0007744\|PDB:5Y1W, ECO:0007744\|PDB:5Y1X, ECO:0007744\|PDB:5Y3I, ECO:0007744\|PDB:6EA1, ECO:0007744\|PDB:6EA2, ECO:0007744\|PDB:6EAA, ECO:0007744\|PDB:6EAB, ECO:0007744\|PDB:6EE3, ECO:0007744\|PDB:6EE4, ECO:0007744\|PDB:6EE6, ECO:0007744\|PDB:6EED, ECO:0007744\|PDB:6SBQ, ECO:0007744\|PDB:6SBR

Chain	Residue	Details
A	HIS496
A	HIS500
A	GLU519

site_id	SWS_FT_FI5
Number of Residues	1
Details	SITE: Important for substrate specificity => ECO:0000269\|PubMed:23897806

Chain	Residue	Details
A	VAL459

site_id	SWS_FT_FI6
Number of Residues	1
Details	SITE: Transition state stabilizer => ECO:0000250\|UniProtKB:P15144

Chain	Residue	Details
A	TYR580

site_id	SWS_FT_FI7
Number of Residues	1
Details	SITE: Cleavage => ECO:0000305\|PubMed:21659511

Chain	Residue	Details
A	ASN795

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)