Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5VPN

E. coli Quinol fumarate reductase FrdA E245Q mutation

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000104	molecular_function	succinate dehydrogenase activity
A	0000166	molecular_function	nucleotide binding
A	0005515	molecular_function	protein binding
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006113	biological_process	fermentation
A	0006974	biological_process	DNA damage response
A	0008177	molecular_function	succinate dehydrogenase (quinone) activity
A	0009055	molecular_function	electron transfer activity
A	0009061	biological_process	anaerobic respiration
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
A	0019645	biological_process	anaerobic electron transport chain
A	0022900	biological_process	electron transport chain
A	0044780	biological_process	bacterial-type flagellum assembly
A	0045284	cellular_component	plasma membrane fumarate reductase complex
A	0050660	molecular_function	flavin adenine dinucleotide binding
A	0071949	molecular_function	FAD binding
B	0005515	molecular_function	protein binding
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006099	biological_process	tricarboxylic acid cycle
B	0006113	biological_process	fermentation
B	0008177	molecular_function	succinate dehydrogenase (quinone) activity
B	0009055	molecular_function	electron transfer activity
B	0009061	biological_process	anaerobic respiration
B	0016020	cellular_component	membrane
B	0016491	molecular_function	oxidoreductase activity
B	0019645	biological_process	anaerobic electron transport chain
B	0044780	biological_process	bacterial-type flagellum assembly
B	0045284	cellular_component	plasma membrane fumarate reductase complex
B	0046872	molecular_function	metal ion binding
B	0051536	molecular_function	iron-sulfur cluster binding
B	0051537	molecular_function	2 iron, 2 sulfur cluster binding
B	0051538	molecular_function	3 iron, 4 sulfur cluster binding
B	0051539	molecular_function	4 iron, 4 sulfur cluster binding
C	0000104	molecular_function	succinate dehydrogenase activity
C	0005886	cellular_component	plasma membrane
C	0006113	biological_process	fermentation
C	0009061	biological_process	anaerobic respiration
C	0016020	cellular_component	membrane
C	0019645	biological_process	anaerobic electron transport chain
C	0044780	biological_process	bacterial-type flagellum assembly
C	0045284	cellular_component	plasma membrane fumarate reductase complex
D	0000104	molecular_function	succinate dehydrogenase activity
D	0005886	cellular_component	plasma membrane
D	0006106	biological_process	fumarate metabolic process
D	0006113	biological_process	fermentation
D	0009061	biological_process	anaerobic respiration
D	0016020	cellular_component	membrane
D	0019645	biological_process	anaerobic electron transport chain
D	0044780	biological_process	bacterial-type flagellum assembly
D	0045284	cellular_component	plasma membrane fumarate reductase complex
E	0000104	molecular_function	succinate dehydrogenase activity
E	0000166	molecular_function	nucleotide binding
E	0005515	molecular_function	protein binding
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0006113	biological_process	fermentation
E	0006974	biological_process	DNA damage response
E	0008177	molecular_function	succinate dehydrogenase (quinone) activity
E	0009055	molecular_function	electron transfer activity
E	0009061	biological_process	anaerobic respiration
E	0016020	cellular_component	membrane
E	0016491	molecular_function	oxidoreductase activity
E	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
E	0019645	biological_process	anaerobic electron transport chain
E	0022900	biological_process	electron transport chain
E	0044780	biological_process	bacterial-type flagellum assembly
E	0045284	cellular_component	plasma membrane fumarate reductase complex
E	0050660	molecular_function	flavin adenine dinucleotide binding
E	0071949	molecular_function	FAD binding
F	0005515	molecular_function	protein binding
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0006099	biological_process	tricarboxylic acid cycle
F	0006113	biological_process	fermentation
F	0008177	molecular_function	succinate dehydrogenase (quinone) activity
F	0009055	molecular_function	electron transfer activity
F	0009061	biological_process	anaerobic respiration
F	0016020	cellular_component	membrane
F	0016491	molecular_function	oxidoreductase activity
F	0019645	biological_process	anaerobic electron transport chain
F	0044780	biological_process	bacterial-type flagellum assembly
F	0045284	cellular_component	plasma membrane fumarate reductase complex
F	0046872	molecular_function	metal ion binding
F	0051536	molecular_function	iron-sulfur cluster binding
F	0051537	molecular_function	2 iron, 2 sulfur cluster binding
F	0051538	molecular_function	3 iron, 4 sulfur cluster binding
F	0051539	molecular_function	4 iron, 4 sulfur cluster binding
G	0000104	molecular_function	succinate dehydrogenase activity
G	0005886	cellular_component	plasma membrane
G	0006113	biological_process	fermentation
G	0009061	biological_process	anaerobic respiration
G	0016020	cellular_component	membrane
G	0019645	biological_process	anaerobic electron transport chain
G	0044780	biological_process	bacterial-type flagellum assembly
G	0045284	cellular_component	plasma membrane fumarate reductase complex
H	0000104	molecular_function	succinate dehydrogenase activity
H	0005886	cellular_component	plasma membrane
H	0006106	biological_process	fumarate metabolic process
H	0006113	biological_process	fermentation
H	0009061	biological_process	anaerobic respiration
H	0016020	cellular_component	membrane
H	0019645	biological_process	anaerobic electron transport chain
H	0044780	biological_process	bacterial-type flagellum assembly
H	0045284	cellular_component	plasma membrane fumarate reductase complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	31
Details	binding site for residue FAD A 601

Chain	Residue
A	GLY11
A	HIS44
A	THR45
A	ALA47
A	ALA48
A	GLY50
A	GLY51
A	HIS155
A	VAL157
A	ALA191
A	THR192
A	ALA12
A	GLY193
A	THR203
A	ASP211
A	LEU242
A	HIS355
A	TYR356
A	GLU379
A	SER393
A	ASN394
A	SER395
A	GLY13
A	LEU396
A	LEU399
A	GLY14
A	ALA15
A	SER36
A	LYS37
A	VAL38
A	SER43

site_id	AC2
Number of Residues	9
Details	binding site for residue FES B 301

Chain	Residue
B	SER56
B	CYS57
B	ARG58
B	ALA60
B	ILE61
B	CYS62
B	GLY63
B	CYS65
B	CYS77

site_id	AC3
Number of Residues	10
Details	binding site for residue F3S B 302

Chain	Residue
B	CYS158
B	CYS204
B	THR205
B	PHE206
B	VAL207
B	GLY208
B	TYR209
B	CYS210
B	ALA221
B	ILE224

site_id	AC4
Number of Residues	10
Details	binding site for residue SF4 B 303

Chain	Residue
B	CYS148
B	ILE149
B	CYS151
B	GLY152
B	LEU153
B	CYS154
B	ALA171
B	CYS214
B	PRO215
B	VAL218

site_id	AC5
Number of Residues	34
Details	binding site for residue FAD E 601

Chain	Residue
E	GLY11
E	ALA12
E	GLY13
E	GLY14
E	ALA15
E	SER36
E	LYS37
E	VAL38
E	SER43
E	HIS44
E	THR45
E	ALA47
E	ALA48
E	GLU49
E	GLY50
E	GLY51
E	HIS155
E	VAL157
E	ALA191
E	THR192
E	GLY193
E	THR203
E	ASP211
E	LEU242
E	HIS355
E	TYR356
E	GLY378
E	GLU379
E	ARG390
E	SER393
E	ASN394
E	SER395
E	LEU396
E	LEU399

site_id	AC6
Number of Residues	8
Details	binding site for residue FES F 301

Chain	Residue
F	GLY63
F	CYS65
F	CYS77
F	CYS57
F	ARG58
F	ALA60
F	ILE61
F	CYS62

site_id	AC7
Number of Residues	9
Details	binding site for residue F3S F 302

Chain	Residue
F	CYS158
F	CYS204
F	THR205
F	PHE206
F	VAL207
F	GLY208
F	TYR209
F	CYS210
F	ALA221

site_id	AC8
Number of Residues	7
Details	binding site for residue SF4 F 303

Chain	Residue
F	CYS148
F	ILE149
F	CYS151
F	GLY152
F	LEU153
F	CYS154
F	CYS214

Functional Information from PROSITE/UniProt

site_id	PS00504
Number of Residues	10
Details	FRD_SDH_FAD_BINDING Fumarate reductase / succinate dehydrogenase FAD-binding site. RSHTvaAeGG

Chain	Residue	Details
A	ARG42-GLY51

site_id	PS00197
Number of Residues	9
Details	2FE2S_FER_1 2Fe-2S ferredoxin-type iron-sulfur binding region signature. CRMAICGSC

Chain	Residue	Details
B	CYS57-CYS65

site_id	PS00198
Number of Residues	12
Details	4FE4S_FER_1 4Fe-4S ferredoxin-type iron-sulfur binding region signature. CiNCGlCYaACP

Chain	Residue	Details
B	CYS148-PRO159

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269\|PubMed:10373108, ECO:0007744\|PDB:1L0V

Chain	Residue	Details
G	ASN65-LEU89
G	GLY104-ALA127
B	TYR13
B	GLN225
F	TYR13
F	GLN225

site_id	SWS_FT_FI2
Number of Residues	22
Details	BINDING: BINDING => ECO:0000269\|PubMed:10373108, ECO:0007744\|PDB:1KF6, ECO:0007744\|PDB:1KFY, ECO:0007744\|PDB:1L0V

Chain	Residue	Details
F	CYS77
F	CYS148
F	CYS151
F	CYS154
F	CYS158
F	CYS204
F	CYS210
F	CYS214
B	CYS204
B	CYS210
B	CYS214
F	CYS57
F	CYS62
F	CYS65
B	CYS57
B	CYS62
B	CYS65
B	CYS77
B	CYS148
B	CYS151
B	CYS154
B	CYS158

site_id	SWS_FT_FI3
Number of Residues	48
Details	TOPO_DOM: Periplasmic => ECO:0000269\|PubMed:10373108

Chain	Residue	Details
D	VAL35-GLN59
H	VAL35-GLN59

site_id	SWS_FT_FI4
Number of Residues	6
Details	TOPO_DOM: Periplasmic => ECO:0000269\|PubMed:10373108, ECO:0000269\|PubMed:15919996

Chain	Residue	Details
D	VAL115-ILE118
H	VAL115-ILE118

site_id	SWS_FT_FI5
Number of Residues	2
Details	BINDING: BINDING => ECO:0007744\|PDB:1L0V

Chain	Residue	Details
D	TRP14
H	TRP14

site_id	SWS_FT_FI6
Number of Residues	2
Details	BINDING: BINDING => ECO:0007744\|PDB:1L0V

Chain	Residue	Details
C	TRP86
G	TRP86

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)