5OGL

Structure of bacterial oligosaccharyltransferase PglB in complex with an acceptor peptide and an lipid-linked oligosaccharide analog

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0004576	molecular_function	oligosaccharyl transferase activity
A	0004579	molecular_function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity
A	0005886	cellular_component	plasma membrane
A	0006486	biological_process	protein glycosylation
A	0016020	cellular_component	membrane
A	0016757	molecular_function	glycosyltransferase activity
A	0018279	biological_process	protein N-linked glycosylation via asparagine
A	0043687	biological_process	post-translational protein modification
A	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue MN A 801

Chain	Residue
A	ASP56
A	ASP154
A	GLU319
A	HOH902
A	HOH903
A	HOH910

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site_id	AC2
Number of Residues	5
Details	binding site for residue MN A 802

Chain	Residue
A	ASP475
A	HOH906
A	ARG65
A	GLN73
A	ASP76

---

site_id	AC3
Number of Residues	11
Details	binding site for residue 9UB A 803

Chain	Residue
A	ASP56
A	TYR79
A	TYR196
A	SER198
A	GLN289
A	TYR293
A	MET368
A	ARG375
A	PHE376
A	TYR379
A	TYR468

---

site_id	AC4
Number of Residues	1
Details	binding site for residue NA A 804

Chain	Residue
A	GLU43

---

site_id	AC5
Number of Residues	7
Details	binding site for Ligand residues PPN B 16 through GLY B 17 bound to THR B 15

Chain	Residue
A	GLU315
A	THR316
A	ILE317
A	MET318
A	ASN321
A	VAL575
B	THR15

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	34
Details	TOPO_DOM: Cytoplasmic => ECO:0000305|PubMed:21677752

Chain	Residue	Details
A	MET1-SER12
A	TYR125-LEU127
A	LYS177-ASP178
A	HIS218-LYS222
A	LYS257-ASN262
A	LYS351-SER355
A	GLU400-SER407

---

site_id	SWS_FT_FI2
Number of Residues	249
Details	TRANSMEM: Helical => ECO:0000269|PubMed:21677752

Chain	Residue	Details
A	ILE13-TRP37
A	PHE99-GLU124
A	THR128-ASN146
A	THR155-ASN176
A	ILE179-TRP194
A	TYR200-PHE217
A	ILE223-LEU235
A	TRP240-PHE256
A	PHE263-SER280
A	PHE328-LEU350
A	MET356-ALA372
A	THR377-LEU399
A	LEU408-TYR432

---

site_id	SWS_FT_FI3
Number of Residues	402
Details	TOPO_DOM: Periplasmic => ECO:0000305|PubMed:21677752

Chain	Residue	Details
A	ALA38-SER98
A	ARG147-ASP154
A	TRP195-SER199
A	SER236-ALA239
A	GLY281-VAL327
A	GLY373-PHE376
A	TYR433-ARG712

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	BINDING: BINDING => ECO:0000269|PubMed:21677752

Chain	Residue	Details
A	ASP56
A	ASP154
A	GLU319

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:29058712

Chain	Residue	Details
A	TYR468
A	TYR196
A	TYR293
A	ARG375

---

site_id	SWS_FT_FI6
Number of Residues	3
Details	SITE: Interacts with target acceptor peptide in protein substrate => ECO:0000269|PubMed:21677752

Chain	Residue	Details
A	ASP56
A	GLU319
A	ILE572

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	SITE: Important for catalytic activity => ECO:0000305|PubMed:21677752

Chain	Residue	Details
A	ARG147

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site_id	SWS_FT_FI8
Number of Residues	1
Details	SITE: Interacts with target acceptor peptide in protein substrate; important for extended sequon recognition => ECO:0000269|PubMed:21677752

Chain	Residue	Details
A	ARG331

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site_id	SWS_FT_FI9
Number of Residues	2
Details	CARBOHYD: N-linked (DATDGlc) asparagine => ECO:0000269|PubMed:21677752

Chain	Residue	Details
A	GLN535
A	PRO556

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